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| Status |
Public on Dec 08, 2022 |
| Title |
Identification and N6-Methyladenosine Modification of the different genes in the peri-infarct cortex of PT mice response to circSCMH1 using MeRIP-seq. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Whole MeRIP-sequencing analysis was performed and analyzed by Lc. Aksomics Co. Ltd. (Shanghai, China). The peri-infarct cortex from sham mice, PT mice with EV-Vector, and PT mice with EV-circSCMH1 was collected in TRIzol. Total RNAs were qualified by agarose gel electrophoresis and quantified using Nanodrop. mRNA was isolated and then fragmented to 100-nucleotide-long fragments. m6A methylated mRNAs were enriched with anti-N6-methyadenosine(m6A) antibody. We use commercial kit for RNA-seq library prepa ration of m6A mRNA and input samples. Completed libraries were qualified and then sequenced on Hiseq 4000 platform.
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| Overall design |
Examination of N6-Methyladenosine Modification by Over-expressed circSCMH1 in the peri-infarct cortex of PT mice by MeRIP-seq.
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| Contributor(s) |
Li B, Yao H |
| Citation(s) |
36717587 |
| |
| Submission date |
Jan 13, 2022 |
| Last update date |
Feb 10, 2023 |
| Contact name |
bin li |
| E-mail(s) |
230198338@seu.edu.cn
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| Organization name |
Southeast University
|
| Street address |
No.87 of Dingjiaqiao
|
| City |
Nanjing |
| ZIP/Postal code |
210000 |
| Country |
China |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA796980 |
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