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| Status |
Public on Jan 05, 2022 |
| Title |
Transcriptome sequencing of bristol strain (wild-type), metl-9 KO strain and metl-9 mutation strain upon P.aeruginosa infection |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We challenge bristol strain (wild-type), metl-9 KO strain (short as KO, has a 101bp insertion, leads to a truncated protein of 258aa) and metl-9 catalytic-activity mutated strain (short as mut, has N172K, D274G mutations in full-length protein) with P.aeruginosa (P.A14), and observe a discrepant transcriptome pattern between wild-type and KO/mut strains. Plenty of innate immune response genes show different expression patterns upon P.A14 infection between the wild-type strain and KO/mut strain. It indicates the important role of metl-9 and 6mA in worm innate immune response modulation.
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| |
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| Overall design |
RNA-seq of wild-type, KO or mut strain upon P.A14 infection, or normal condition (OP50).
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| Contributor(s) |
Ma C, Guan J, Ding W, Li C |
| Citation missing |
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| |
| Submission date |
Jan 02, 2022 |
| Last update date |
Jan 06, 2022 |
| Contact name |
Guan Jialiang |
| E-mail(s) |
guanjialiang@pku.edu.cn
|
| Phone |
18800192720
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| Organization name |
Peking University
|
| Street address |
China, Beijing, Haidian district, Peking University, 1000871
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| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100871 |
| Country |
China |
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| Platforms (1) |
| GPL30091 |
DNBSEQ-G400 (Caenorhabditis elegans) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA793702 |
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