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| Status |
Public on Dec 31, 2023 |
| Title |
Identification of TBX3 and shared HAND2-TBX3 target genes in early mouse limb buds [ChIP-seq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
ChIP-seq is combined with RNA-seq analysis to identify the TBX3 and HAND2 target genes during mouse limb bud development. This analysis identifies the DEGs and direct transcriptional targets of HAND2 and TBX3 during the early determinative period critical to establishment of limb axis polarity and the SHH signaling center in the posterior limb bud mesenchyme. In particular, bioinformatics analysis identifies the target gene networks that are co-regulated by both TBX3 and HAND2 transcription factors in the limb bud mesenchyme. A significant fraction of the target genes identified are required for normal limb bud development and their spatio-temporal expression patterns are changed in mutant limb buds at early stages. This project was supported by SNSF Grant 310030_184734 to Rolf Zeller (project partner: Aimée Zuniga).
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| Overall design |
Identification of the genomic regions enriched in TBX3 chromatin complexes in mouse limb buds using ChIP-seq
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| |
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| Contributor(s) |
Jhanwar S, Sheth R, Zuniga A, Zeller R |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Dec 22, 2021 |
| Last update date |
Dec 31, 2023 |
| Contact name |
Aimee Zuniga |
| E-mail(s) |
devgenlab-dbm@unibas.ch
|
| Organization name |
University of Basel
|
| Department |
Department of Biomedicine
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| Lab |
Developmental Genetics
|
| Street address |
Mattenstrasse, 28 4058
|
| City |
Basel |
| ZIP/Postal code |
4058 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (4)
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| GSM5747747 |
ChIP Tbx3 FL bud E9.75-E10.25 replicate 1 |
| GSM5747748 |
Input Tbx3 FL bud E9.75-E10.25 replicate 1 |
| GSM5747749 |
ChIP Tbx3 FL bud E9.75-E10.25 replicate 2 |
| GSM5747750 |
Input Tbx3 FL bud E9.75-E10.25 replicate 2 |
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| This SubSeries is part of SuperSeries: |
| GSE192486 |
Identification of TBX3 and shared HAND2-TBX3 target genes in early mouse limb buds |
|
| Relations |
| BioProject |
PRJNA791649 |
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