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| Status |
Public on Dec 05, 2009 |
| Title |
Identification of Sfh and H-NS binding sites in the Salmonella Typhimurium genome |
| Organism |
Salmonella enterica subsp. enterica serovar Typhimurium |
| Experiment type |
Genome binding/occupancy profiling by array
|
| Summary |
The global regulator H-NS represses transcription in gram negative bacteria. Sfh is a homologue of H-NS and is encoded by plasmid pSfR27. Sfh provides a 'stealth' function that allows pSfR27 to be transmitted to a new host without disrupting the competitive fitness of the new host
We used ChIP-on-chip to profile Sfh (3xFLAG-tagged) and H-NS binding sites in Salmonella Typhimurium strain SL1344 and found that Sfh provides its 'stealth' function by targeting a sub-set of H-NS bound genes that display reduced levels of H-NS occupancy with the SL1344 chromosome upon acquisition of plasmid pSfR27
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| Overall design |
Identification of Sfh binding sites in strains SL1344 (pSfR27) and SL1344 hns (pSfR27) as well as identification of H-NS binding sites in strains SL1344, SL1344 (pSfR27), and SL1344 (pSfR27) sfh
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| Contributor(s) |
Dillon SC, Cameron AD, Hokamp K, Lucchini S, Hinton JC, Dorman CJ |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Nov 30, 2009 |
| Last update date |
Mar 21, 2012 |
| Contact name |
Shane Dillon |
| E-mail(s) |
scdillon@tcd.ie
|
| Organization name |
Moyne Institute of Preventive Medicine
|
| Department |
Department of Microbiology
|
| Street address |
Trinity College
|
| City |
Dublin |
| ZIP/Postal code |
Dublin 2 |
| Country |
Ireland |
| |
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| Platforms (1) |
| GPL5791 |
IFR OGT Salmonella SL1344 22k v1.0 |
|
| Samples (8)
|
| GSM476560 |
Sfh 3xFLAG SL1344 pSfR27 BR1 |
| GSM476561 |
Sfh 3xFLAG SL1344 pSfR27 BR2 |
| GSM476562 |
Sfh 3xFLAG SL1344 hns minus pSfR27 biological rep 1 |
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| This SubSeries is part of SuperSeries: |
| GSE19356 |
Sfh and H-NS binding sites in the Salmonella Typhimurium genome |
|
| Relations |
| BioProject |
PRJNA123763 |
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