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| Status |
Public on Nov 13, 2022 |
| Title |
Transcriptomes of cultured human hematopoietic stem cells. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We compared transcriptomes of CD34(high)EPCR-positive with CD34(high)EPCR-negative cells after 10-days culture in PCL-PVAc-PEG based 3a medium.
It was because our culture condition had not previously reported and so we demonstrated that phenotypic HSCs were concentrated in EPCR-positive fraction as conventional UM171-based culture system.
As a result, CD34(high)EPCR-positive cells highly expressed HSC markers (HLF, AVP, PRDM16 and FGD5) compared with CD34(high)EPCR-negative cells.
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| |
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| Overall design |
mRNA profile of CD34(high)EPCR-positive and CD34(high)EPCR-negative cells derived from 10-days cultured cord blood CD34+ cells.
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| |
|
| Contributor(s) |
Sakurai M, Ishitsuka K |
| Citation(s) |
36813966 |
| Submission date |
Dec 21, 2021 |
| Last update date |
Mar 03, 2023 |
| Contact name |
Masatoshi Sakurai |
| Organization name |
The Institute of Medical Science, The University of Tokyo
|
| Department |
Stem Cell Biology and Regenerative Medicine
|
| Lab |
Stem Cell Biology
|
| Street address |
6-1
|
| City |
4 Shiroganedai, Minato-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
108-8639 |
| Country |
Japan |
| |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (6)
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| Relations |
| BioProject |
PRJNA791215 |
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