 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Apr 01, 2022 |
| Title |
Bone Marrow - scRNA-sequencing |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The goal of this experiment was to address when during development, deletion of the locus control region 1 (LCR1), impact the development of ILC2 in the bone marrow.
|
| |
|
| Overall design |
In order to determine consequences of LCR1 deletion on ILC2 development, 8-12 weeks old WT and LCR1-/- C57BL/6J mice were sacrified and BM cells were extracted by crushing bones. Following BM cell extraction, ACK lysis for performed to remove red blood cell. Cells were filtered through a 70uM strainer and sequentially with viability dye and flow cytometry antibodies (in FACS buffer). Cells were then filtered again and sorted using a FACS ARIA II (BD). Viable, CD45+, Lineage-, CD127+ cells were sorted with a purity >90% then prepared for 10X Genomics scRNAsequencing experiment. scRNAseq experiment was performed following manufacturer's recommendations.
|
| |
|
| Contributor(s) |
Michieletto MF, Tello-Cajiao JJ, Mowel WK, Chandra A, Yoon S, Joannas L, Clark ML, Jimenez MT, Wright JM, Lundgren P, Williams A, Thaiss CA, Vahedi G, Henao-Mejia J |
| Citation(s) |
36050414 |
| |
| Submission date |
Dec 20, 2021 |
| Last update date |
Sep 07, 2022 |
| Contact name |
John James Tello-Cajiao |
| Organization name |
university of Pennsylvanya
|
| Department |
Genetics
|
| Lab |
Henao-Mejia
|
| Street address |
Curie Blvd 421
|
| City |
Philadelphia |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (2) |
|
| This SubSeries is part of SuperSeries: |
|
| Relations |
| BioProject |
PRJNA790965 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE191308_RAW.tar |
101.3 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
