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Series GSE191193 Query DataSets for GSE191193
Status Public on Apr 19, 2023
Title Single-cell RNA-sequencing reveals an intratumor heterogeneity in pancreatic adenocarcinoma [scRNA-seq]
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We examined the intratumor heterogeneity in S2-VP10 xenograft by single-cell RNA-sequencing. We found a hierarchical tumor progression originating from ROR1high cells with a partial EMT gene signature. We also found that ROR1high cells have the strong capacity as tumor-initiating cells to support tumorigenecity, chemoresistance, and metastasis. CUT&RUN and ATAC-sequencing revealed that ROR1 gene expression is regulated through YAP-BRD4 axis. Together, these data uncover a role for ROR1high cells in tumor progression, suggesting that ablating ROR1high tumor-initiating cells may be the alternative therapeutic strategy in pancreatic adenocarcinoma.
 
Overall design Examination of single-cell RNA sequencing using 10X genomics chromium in S2-VP10 xenograft.
 
Contributor(s) Yamazaki M
Citation(s) 37096681
Submission date Dec 18, 2021
Last update date May 01, 2023
Contact name Masaya Yamazaki
E-mail(s) myamazaki@kumamoto-u.ac.jp
Organization name Kumamoto university
Lab Medical Biochemistry
Street address 1-1-1, Honjyo, Chuo-ku
City Kumamoto city
ZIP/Postal code 860-8556
Country Japan
 
Platforms (1)
GPL20795 HiSeq X Ten (Homo sapiens)
Samples (1)
GSM5741306 S2-VP10_xenograft_Chromium
This SubSeries is part of SuperSeries:
GSE191204 Single-cell RNA-sequencing reveals an intratumor heterogeneity in pancreatic adenocarcinoma
Relations
BioProject PRJNA790077

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE191193_RAW.tar 67.2 Mb (http)(custom) TAR (of TAR)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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