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Series GSE19116 Query DataSets for GSE19116
Status Public on Apr 09, 2010
Title Xylose utilization timecourse
Organism Saccharomyces cerevisiae
Experiment type Expression profiling by array
Summary We have identified a novel xylose-utilization phenotype controlled by a single gene, and we sought to characterize how both the presence and absence of xylose in the presence or absence of the trait affected the S. cerevisiae transcriptional program over time. To do so, we measured global steady state mRNA levels on three pairs of sister spores from a Simi White strain that was backcrossed twice to S288c. Each pair of spores was from an independent tetrad, and contained one xylose utilizing ("positive", GSY2465, 2466, 2469) and one xylose non-utilizing spore ("negative", GSY2464, 2467, 2470). We grew each of the six spores in the presence or absence of xylose for 72 hours with samples taken every 8 hours. We then assayed relative RNA abundance versus a pooled reference, containing equimolar amounts of each sample, using Agilent yeast catalog arrays.

Overall design time_series_design
Contributor(s) Wenger J
Citation(s) 20485559
Submission date Nov 20, 2009
Last update date Mar 21, 2012
Contact name Gavin Sherlock
Phone 650 498 6012
Fax 650 724 3701
Organization name Stanford University
Department Genetics
Street address 300 Pasteur Drive
City Stanford
State/province CA
ZIP/Postal code 94305-5120
Country USA
Platforms (1)
GPL9703 Agilent yeast array
Samples (135)
GSM473911 JWY5_nc_t56
GSM473912 JWY5_xylose_t0
GSM473913 JWY1_xylose_t16
This SubSeries is part of SuperSeries:
GSE19121 Xylose Utilization Gene in Saccharomyces cerevisiae
BioProject PRJNA123739

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE19116_RAW.tar 1.5 Mb (http)(custom) TAR
Raw data included within Sample table
Processed data included within Sample table

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