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| Status |
Public on Apr 27, 2023 |
| Title |
Imbalanced gut microbiota drives nonalcoholic fatty liver disease and nonalcoholic steatohepatitis in a fast-food diet mouse model |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
This study employed low and high-fat purified diets containing two levels of added dietary cholesterol in conventionally raised, specific-pathogen free (SPF) mice harboring a full microbial community as compared to germ-free (GF) mice raised in complete absence of gut microbes on disease outcomes. It was hypothesized that FF-diets would lead to an earlier onset of gut dysbiosis, corresponding with elevated biomarkers of NAFLD and NASH. In the present study, we aimed to dissect the role of FF diet induced gut dysbiosis in order to mechanistically identify the underlying mechanism that influences gut-liver crosstalk between specific diet-induced bacterial populations and hepatic tissue to drive NAFLD/NASH onset and progression
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| Overall design |
Germ-free and SPF mice were fed one of four purified diets for either 8 or 24 weeks and saccrificed. NAFLD/NASH progression was evaluated via histology and biochemical analyses and concomitant changes in gut microbiota was assessed via 16S rRNA gene amplicon sequencing and shotgun metagenomics.
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| Contributor(s) |
Fei N, Miyoshi S, Hermanson J, Hawkins M, Miyoshi J, Charlton W, Xie B, D'Sousa M, Sulakhe D, Chang EB, Charlton M, Leone V |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Dec 15, 2021 |
| Last update date |
Apr 27, 2023 |
| Contact name |
Vanessa Leone |
| E-mail(s) |
valeone@wisc.edu
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| Organization name |
University of Wisconsin-Madison
|
| Street address |
1933 Observatory Dr.
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53706 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (67)
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| Relations |
| BioProject |
PRJNA789248 |
| SRA |
SRP351336 |
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