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| Status |
Public on Jan 07, 2022 |
| Title |
Recruitment of highly cytotoxic CD8+ T cell receptors in mild SARS-CoV-2 infection |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
T cell immunity is crucial for the control of SARS-CoV-2 infections and has been widely studied on a quantitative level. However, quality of responses, in particular of CD8+ T cells, has only been marginally investigated so far. Here, we isolated T cell receptor (TCR) repertoires specific for immunodominant SARS-CoV-2 epitopes restricted to common Human Leucocyte Antigen (HLA) class I molecules in convalescent individuals. SARS-CoV-2-specific CD8+ T cells were detected up to twelve months from infection. TCR repertoires were diverse, with heterogeneous functional avidity and cytotoxicity towards virus-infected cells, as demonstrated for TCR-engineered T cells. High TCR functionality correlated with gene signatures that, remarkably, could be retrieved for each epitope:HLA combination analyzed. Overall, our data demonstrate that polyclonal and highly functional CD8+ TCRs – classical features of protective immunity - are recruited upon mild SARS-CoV-2 infection, providing tools to assess the quality and to potentially restore functional CD8+ T cell immunity.
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| Overall design |
scRNA sequencing data for single SARS-CoV-2 peptide-stimulated CD8 T cell samples from convalescent donors
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| Contributor(s) |
Wagner KI, Mateyka LM, Jarosch S, Grass V, Weber S, Schober K, Hammel M, Burrell T, Kalali B, Poppert H, Beyer H, Schambeck S, Holdenrieder S, Strötges-Achatz A, Haselmann V, Neumaier M, Erber J, Priller A, Yazici S, Roggendorf H, Odendahl M, Tonn T, Dick A, Witter K, Mijočević H, Protzer U, Knolle PA, Pichlmair A, Crowell CS, Gerhard M, D’Ippolito E, Busch DH |
| Citation(s) |
34968416 |
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| Submission date |
Dec 13, 2021 |
| Last update date |
Jan 08, 2022 |
| Contact name |
Sebastian Jarosch |
| E-mail(s) |
sebastian.jarosch@tum.de
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| Organization name |
TU Munich
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| Department |
Institute for Medical Microbiology, Immunology and Hygiene
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| Lab |
Prof. Dirk Busch
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| Street address |
Trogerstr. 30
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| City |
Munich |
| State/province |
Bavaria |
| ZIP/Postal code |
81675 |
| Country |
Germany |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (8)
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| GSM5732740 |
Experiment_1_donor_24_34_A3_ORF1_VTN_GEX |
| GSM5732741 |
Experiment_1_donor_24_34_A3_ORF1_VTN_VDJ |
| GSM5732742 |
Experiment_1_donor_32_33_A1_ORF3a_FTS_GEX |
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| Relations |
| BioProject |
PRJNA788547 |
| SRA |
SRP350830 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE190839_Experiment_1_metadata.csv.gz |
1.2 Mb |
(ftp)(http) |
CSV |
| GSE190839_Experiment_2_metadata.csv.gz |
849.5 Kb |
(ftp)(http) |
CSV |
| GSE190839_RAW.tar |
310.2 Mb |
(http)(custom) |
TAR (of JSON, MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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