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Series GSE19082 Query DataSets for GSE19082
Status Public on Nov 19, 2009
Title IL6 - IFNγ crosstalk experiment
Organism Homo sapiens
Experiment type Expression profiling by array
Summary This experiment tested whether pretreatment of human microvascular endothelial cells (HMECs) with interferon-gamma (IFNγ) for 2h affected the transcriptional response to 1h exposure to interleukin-6 (IL-6).
 
Overall design Human microvascular endothelial cells in culture; exposure to IFNγ 1 ng/ml for 3 h alone, with IFNγ 1 ng/ml for 2 h and then with IL6 50 U/ml for 1 h, with IL6 50 U/ml alone, or with neither. 4 replicates for each condition. RNA isolation, Illumina expression arrays.

The supplementary file 'GSE19082_non-normalized_data.txt' contains non-normalized data for Samples GSM472434-GSM472449.
 
Contributor(s) Braam B, Rastmanesh M, Bluyssen H, Jie K, Wesseling S
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Nov 18, 2009
Last update date Jan 18, 2013
Contact name Branko Braam
E-mail bbraam@gmail.com
Phone 780 407 3322
Fax 780 407 7771
URL http://www.nephrogenomics.net
Organization name Univ. of Alberta
Department Medicine / Nephrology
Street address 11-107 CSB
City Edmonton
ZIP/Postal code T6C 3X6
Country Canada
 
Platforms (1)
GPL6104 Illumina humanRef-8 v2.0 expression beadchip
Samples (16)
GSM472434 IFN-gamma 0ng/ml 2h + IL-6 0U/ml 1h CT ExpC05
GSM472435 IFN-gamma 0ng/ml 2h + IL-6 50U/ml 1h CT ExpC05
GSM472436 IFN-gamma 1ng/ml 2h + IL-6 0U/ml 1h CT ExpC05
Relations
BioProject PRJNA120493

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE19082_RAW.tar 170.3 Mb (http)(custom) TAR (of TXT)
GSE19082_non-normalized_data.txt.gz 1.5 Mb (ftp)(http) TXT
Raw data provided as supplementary file
Processed data included within Sample table
Raw data is available on Series record

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