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Series GSE19082 Query DataSets for GSE19082
Status Public on Nov 19, 2009
Title IL6 - IFNγ crosstalk experiment
Organism Homo sapiens
Experiment type Expression profiling by array
Summary This experiment tested whether pretreatment of human microvascular endothelial cells (HMECs) with interferon-gamma (IFNγ) for 2h affected the transcriptional response to 1h exposure to interleukin-6 (IL-6).
Overall design Human microvascular endothelial cells in culture; exposure to IFNγ 1 ng/ml for 3 h alone, with IFNγ 1 ng/ml for 2 h and then with IL6 50 U/ml for 1 h, with IL6 50 U/ml alone, or with neither. 4 replicates for each condition. RNA isolation, Illumina expression arrays.

The supplementary file 'GSE19082_non-normalized_data.txt' contains non-normalized data for Samples GSM472434-GSM472449.
Contributor(s) Braam B, Rastmanesh M, Bluyssen H, Jie K, Wesseling S
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Nov 18, 2009
Last update date Jan 18, 2013
Contact name Branko Braam
Phone 780 407 3322
Fax 780 407 7771
Organization name Univ. of Alberta
Department Medicine / Nephrology
Street address 11-107 CSB
City Edmonton
ZIP/Postal code T6C 3X6
Country Canada
Platforms (1)
GPL6104 Illumina humanRef-8 v2.0 expression beadchip
Samples (16)
GSM472434 IFN-gamma 0ng/ml 2h + IL-6 0U/ml 1h CT ExpC05
GSM472435 IFN-gamma 0ng/ml 2h + IL-6 50U/ml 1h CT ExpC05
GSM472436 IFN-gamma 1ng/ml 2h + IL-6 0U/ml 1h CT ExpC05
BioProject PRJNA120493

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE19082_RAW.tar 170.3 Mb (http)(custom) TAR (of TXT)
GSE19082_non-normalized_data.txt.gz 1.5 Mb (ftp)(http) TXT
Raw data provided as supplementary file
Processed data included within Sample table
Raw data is available on Series record

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