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| Status |
Public on Aug 30, 2022 |
| Title |
RBM45 is an m6A-binding protein that affects neuronal differentiation and the splicing of a subset of pre-mRNAs IV |
| Organism |
Mus musculus |
| Experiment type |
Other
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| Summary |
Here, we identify RNA-binding motif containing protein 45 (RBM45) as a novel m⁶A reader, enriched in the brain, and highly expressed during embryonic neurodevelopment. We demonstrate that RBM45 recognizes intronic m⁶A and that its primary role is as a direct regulator of pre-mRNA splicing for its target genes, many of which are associated with neurodevelopment. Furthermore, we show that RBM45 is absolutely required for proper differentiation in cultured SH-SY5Y neuroblastoma cells, and that RBM45 regulates splicing of neurodevelopmental target genes central to the activation of gene expression programs that regulate differentiation in vitro.
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| Overall design |
in vitro DART-seq m⁶A profiling on subcellular fractionated RNAs (chromatin associated (CA), nuclear, and cytoplasmic RNA) from immortalized mouse hippocampal neurons (mHiPPoE-2)
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| Contributor(s) |
Choi SH, Zhu H, Flamand M, Meyer KD |
| Citation(s) |
36044854 |
| |
| Submission date |
Dec 06, 2021 |
| Last update date |
Sep 27, 2022 |
| Contact name |
Kate Meyer |
| E-mail(s) |
kate.meyer@duke.edu
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| Phone |
9196849562
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| Organization name |
Duke University School of Medicine
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| Department |
Biochemistry
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| Street address |
307 Research Drive Box 3711
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| City |
D |
| State/province |
NC |
| ZIP/Postal code |
27710 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE190324 |
RBM45 is an m6A-binding protein that affects neuronal differentiation and the splicing of a subset of pre-mRNAs |
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| Relations |
| BioProject |
PRJNA786624 |
| SRA |
SRP349492 |
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