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| Status |
Public on Jan 27, 2022 |
| Title |
Gpr125 is a Unifying Hallmark of Multiple Mammary Progenitors coupled with Tumor Latency |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Several markers have been used to identify mammary stem cell subpopulations. However, a specific unifying hallmark of progenitors has not yet been identified yet. Here we show the orphan adhesion G-protein coupled receptor, Gpr125, identifies and locates long-lived progenitors at multiple sites and stages of mammary development. In particular, Gpr125 is expressed in disparate cell populations with documented regenerative capacity in the nipple-proximal and in the TEB-distal end of the mammary gland. To compare these two major Gpr125 cell populations we used whole RNAseq genome to characterize their gene expression profile. Our data revealed Gpr125+ cells at the nipple-proximal end of the gland are engaged in chemorepulsion and the formation of cell surface structures associated with cell movement. In contrast Gpr125+ cells at the TEB-distal tips exhibit a hybrid epithelial-mesenchymal phenotype and are equipped to bind chemokine and growth factors and secrete a promigratory matrix.
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| Overall design |
We performed whole genome RNAseq on Gpr125+ (FDG+/CD49hi) and Gpr125- (FDG-/ CD49hi) cell population isolated from TEB-distal and nipple-proximal mammary regions of pubertal mice Adgra3-Lz/+ mice. Mammary glands were harvested at puberty. Both abdominal mammary glands from 5 mice were pooled together per each sample used in the study. The lymph nodes were removed and their position was used to cut the mammary gland into distal and nipple proximal halves. The mammary glands were mechanically dissociated and digested using a mix consisting of 1 mL Collagenase/Hyaluronidase and 9 mL Epicult-B Basal Medium and incubated for 6-8 hours at 37° C. Cells that were positive for CD31, CD45 or Ter119 were first excluded. Cells were sorted based on their incorporation of fluorescein di-V-galactoside (FDG) and surface expression of CD49fhi.
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| Contributor(s) |
Spina E, Cowin P |
| Citation(s) |
35302059 |
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| Submission date |
Dec 04, 2021 |
| Last update date |
Apr 06, 2022 |
| Contact name |
Ziyan Lin |
| E-mail(s) |
ziyanlin2352@gmail.com
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| Organization name |
NYU Grossman School of Medicine
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| Department |
Applied Bioinformatics Laboratories
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| Street address |
227 E 30th St.
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10016 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA786219 |
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