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| Status |
Public on Apr 30, 2022 |
| Title |
Targeted DamID to understand transcription factor genomic occupancy in human foetal lung progenitor cells |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
In order to study the genomic occupancy of transcription factors, including SOX9, ETV4 and ETV5, in human foetal lung tip progenitor cells, we have introduced Targeted DamID system via lentivirus in a human foetal lung tissue derived organoid culture (Nikolic et al., 2017). We were able to identify the corresponding TF motif enrichment and discovered a potential co-regulation function of SOX9 and ETV factors in human feotal lung progenitor cells. This study has pioneered the use of targeted DamID approach in tissue derived organoid system.
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| Overall design |
We used 4 different independent organoid lines treated as replicates, all of which are around 8 pcw. We introduced the SOX9 targeted DamID into organoids via lentiviral transduction and compared with the results from organoids with Dam only controls. In a following-up experimental setting, we introduced ETV4 and ETV5 targeted DamID into organoids via lentiviral transduction and compared with the results from organoids with Dam only controls.
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| Contributor(s) |
Sun D, Llorà-Batlle O, Van Den Ameele J, Brand AH, Rawlins EL |
| Citation(s) |
36121125 |
| |
| Submission date |
Nov 29, 2021 |
| Last update date |
Oct 01, 2022 |
| Contact name |
Dawei Sun |
| E-mail(s) |
sundawei45232@gmail.com
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| Organization name |
University of Cambridge
|
| Department |
The Gurdon Institute
|
| Street address |
Tennis Court Road
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 1QN |
| Country |
United Kingdom |
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| Platforms (2) |
| GPL18460 |
Illumina HiSeq 1500 (Homo sapiens) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (20)
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| This SubSeries is part of SuperSeries: |
| GSE189885 |
Human fetal lung tip progenitor cells |
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| Relations |
| BioProject |
PRJNA784520 |
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