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| Status |
Public on May 16, 2022 |
| Title |
mRNA-seq analysis of mouse hepatocellular carcinoma cell lines |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
There are few well-characterized syngeneic murine models for hepatocellular carcinoma (HCC), which limits immunological studies and the development of immunotherapies for HCC. We previously established an oncogene-induced spontaneous HCC mouse model based on transposon-mediated oncogene (AKT and NRASG12V) insertion into the genome of hepatocytes to induce tumorigenesis. Here is the result of transcriptomic analysis of two pool cell clones (pool #1 and pool #8) and three single cell clones (NHRI-1-F1, NHRI-8-A4 and NHRI-8-C4) from Akt/N-Ras-induced HCC mouse model, also including a commercial murine hepatocellular carcinoma cell line (Hepa 1-6) as control.
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| Overall design |
Before cells reaching confluence, total RNA was extracted from each cell clone by using RNeasy mini kit (QIAGEN, Hilden, Germany). The quality of RNA was validated by a Bioanalyzer (Agilent, Santa Clara, CA, USA) and subjected to Welgene Biotech Co., Ltd. for RNA-sequencing. Clean reads of each sample provided by the sequencing Labs were used for downstream bioinformatic analysis.
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| Contributor(s) |
Ko Y, Cheng Y, Huang L |
| Citation missing |
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| Submission date |
Nov 23, 2021 |
| Last update date |
May 16, 2022 |
| Contact name |
Li-Rung Huang |
| E-mail(s) |
lrhuang@nhri.edu.tw
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| Organization name |
National Health Research Institutes
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| Street address |
No.35, Keyan rd. Zhunan town
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| City |
Miaoli County |
| ZIP/Postal code |
35053 |
| Country |
Taiwan |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA782911 |
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