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Series GSE189286 Query DataSets for GSE189286
Status Public on Jul 27, 2022
Title Liver ILC2s suppress gluconeogenesis and limit blood glucose elevation through IL-13 signaling [scRNA-seq_Hepatocyte]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The liver stores glycogen and releases blood glucose and is therefore essential for glucose metabolism. Group 2 innate lymphoid cells (ILC2s) in adipose and pancreatic tissues are involved in glucose homeostasis, but the metabolic contribution of liver ILC2s remains unclear. Herein, we show that interleukin (IL)-33 treatment induced IL-13 production in liver ILC2s, which consequently reduced blood glucose levels. IL-13 also directly suppressed gluconeogenesis in primary hepatocytes. Single-cell RNA sequencing (scRNA-seq) and cell-cell interaction analysis in liver ILC2s and hepatocytes demonstrated that IL-33 administration suppressed gluconeogenesis in a specific Hnf4a/G6pchigh-hepatocyte cluster involving expression of Stat3, which significantly interacted with liver ILC2s via IL-13/IL-13 receptor signaling. To address the regulatory mechanism underlying IL-13 production in liver ILC2s, we performed GATA3 transcriptional complex analysis, and GATA3-ChIP-seq, ATAC-seq, and scRNA-seq trajectory analyses, focusing on GATA3-interacting proteins that were functionally specific in ILC2s. AP-1 family members were found to suppress the induction of IL-13 in liver ILC2s. Thus, our study revealed a novel role for liver ILC2s in glucose homeostasis.
 
Overall design Gene expression levels of primary hepatocyte from PBS-treated or IL-33 treated mice were evaluted using single cell RNA-seq.
 
Contributor(s) Fujimoto M, Eunyoung L, Hatano R, Miki T, Tanaka T
Citation(s) 36109558
Submission date Nov 21, 2021
Last update date Sep 27, 2022
Contact name Masanori Fujimoto
E-mail(s) fujimoto212@gmail.com
Organization name Chiba University
Department Graduate School of Medicine
Lab 1. Department of Molecular Diagnosis, 2. Department of Endocrinology, Hematology and Gerontology
Street address Inohana 1-8-1
City Chiba-city
State/province Chiba-prefecture
ZIP/Postal code 260-8677
Country Japan
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (2)
GSM5697026 HepatocyteIL33
GSM5697027 HepatocytePBS
This SubSeries is part of SuperSeries:
GSE189287 Liver ILC2s suppress gluconeogenesis and limit blood glucose elevation through IL-13 signaling
Relations
BioProject PRJNA782349
SRA SRP347118

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE189286_RAW.tar 99.6 Mb (http)(custom) TAR (of TAR)
GSE189286_SupplementaryTable3_AllMarkers_2.csv.gz 149.0 Kb (ftp)(http) CSV
GSE189286_SupplementaryTable4_HepatocyteMarkers.csv.gz 44.2 Kb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record
Processed data provided as supplementary file

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