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| Status |
Public on Sep 06, 2022 |
| Title |
Single-Cell Sequencing Reveals the Overall Changes in Tumor Microenvironment Mediated by CD39i |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Here we identified the ecto-enzyme, CD39 (encoded by ENTPD1), as a potential therapeutic target for BC via single-cell transcriptome analysis of BC and para-cancer tissues. In a subcutaneous model, inhibition of CD39 (CD39i) is able to limit the growth of BC and improve overall survival of tumor-bearing mice. Via single-cell sequencing of tumor infiltrated immune cells, we found that CD39i with POM-1 increased the intratumor NK cells, conventional type 1 dendritic cells (cDC1) and CD8+ T cells, along with decreased Treg abundance. The anti-tumor effect and reprogram of tumor microenvironment are blockade in a cDC1-deficient Batf3−/− model.
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| |
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| Overall design |
Comparing changes in TME caused by CD39i.
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| |
|
| Contributor(s) |
Liu L, Hou Y |
| Citation(s) |
36347860 |
| |
| Submission date |
Nov 18, 2021 |
| Last update date |
Nov 29, 2022 |
| Contact name |
Junyi Hu |
| Organization name |
Union Hospital, Tongji Medical College, Huazhong University of Science and Technology
|
| Department |
Urology
|
| Street address |
No. 1277 Liberation Avenue
|
| City |
Wu Han |
| ZIP/Postal code |
430022 |
| Country |
China |
| |
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| Platforms (1) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA781604 |
| SRA |
SRP346770 |
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