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Series GSE188685 Query DataSets for GSE188685
Status Public on Jun 20, 2022
Title Epigenetic modifier Smchd1 maintains a normal pool of long-term repopulating hematopoietic stem cells in mice II
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We have used a conditional mouse model to investigate the role of Smchd1 specifically in hematopoiesis. We found that Smchd1-deleted mice have an altered hematopoietic stem and progenitor cell (HSPC) compartment with reduced repopulating capacity in competitive bone marrow transplantation assays and the gradual loss of adult hematopoietic stem cells (HSCs) with age, suggesting a role for Smchd1 in the maintenance of long-term adult HSCs. The phenotype was more pronounced in female Smchd1-deleted mice, which showed a more severe reduction in HSCs with age, as well as depleted numbers of B cell lymphocytes. Gene expression profiling of Smchd1 deficient HSCs and B cells revealed several known and novel Smchd1-sensitive genes, a significant disruption to X-linked gene expression, although this was not widespread upregulation of X-linked genes in female cells. These data show Smchd1 as a new regulator of long-term HSCs whose effects are more profound in females.
 
Overall design We have compared Smchd1 deleted B cells with controls that are heterozygous for the same Smchd1 deletion. Here we have compared n=5 female Smchd1 null bone marrow-derived B cells with n=5 female control cells of the same type, and n=4 Smchd1 null male and n=4 control male samples of the same cell type.
 
Contributor(s) Kinkel S, Blewitt M, Hickey PF
Citation(s) 35856023
Submission date Nov 12, 2021
Last update date Aug 02, 2022
Contact name Peter Hickey
E-mail(s) hickey@wehi.edu.au
Organization name WEHI
Street address 1G Royal Parade
City Parkville
ZIP/Postal code 3052
Country Australia
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (18)
GSM5688390 B167_het
GSM5688391 B189_het
GSM5688392 B202_het
This SubSeries is part of SuperSeries:
GSE188686 Epigenetic modifier Smchd1 maintains a normal pool of long-term repopulating hematopoietic stem cells in mice
Relations
BioProject PRJNA779954

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE188685_Smchd1_femBcells_rawcounts.txt.gz 1.5 Mb (ftp)(http) TXT
GSE188685_Smchd1_maleBcells_rawcounts.txt.gz 1.4 Mb (ftp)(http) TXT
GSE188685_female_B_cells.DEGs.csv.gz 2.0 Mb (ftp)(http) CSV
GSE188685_male_B_cells.DEGs.csv.gz 2.0 Mb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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