|
| Status |
Public on Nov 25, 2009 |
| Title |
Genome-wide maps of GATA factor occupancy in K562 cells |
| Project |
ENCODE
|
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
GATA factors interact with simple DNA motifs (WGATAR) to regulate critical processes, including hematopoiesis, but very few WGATAR motifs are occupied in genomes. Given the rudimentary knowledge of mechanisms underlying this restriction, and how GATA factors establish genetic networks, we used ChIP-seq to define GATA-1 and GATA-2 occupancy genome-wide in erythroid cells. Coupled with genetic complementation analysis and transcriptional profiling, these studies revealed a rich collection of targets containing a characteristic binding motif of greater complexity than WGATAR. GATA factors occupied loci encoding multiple components of the Scl/TAL1 complex, a master regulator of hematopoiesis and leukemogenic target. Mechanistic analyses provided evidence for cross-regulatory and autoregulatory interactions among components of this complex, including GATA-2 induction of the hematopoietic corepressor ETO-2 and an ETO-2 negative autoregulatory loop. These results establish fundamental principles underlying GATA factor mechanisms in chromatin and illustrate a complex network of considerable importance for the control of hematopoiesis.
For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf
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| |
|
| Overall design |
Examination of GATA1 and GATA2 occpancy in K562 cells
|
| Web link |
http://www.ncbi.nlm.nih.gov/geo/info/ENCODE.html
|
| |
|
| Contributor(s) |
Fujiwara T, O'Geen H, Keles S, Blahnik K, Linnemann AK, Kang Y, Choi K, Farnham PJ, Bresnick EH |
| Citation(s) |
19941826 |
| BioProject |
PRJNA63447 |
| |
| Submission date |
Nov 03, 2009 |
| Last update date |
May 15, 2019 |
| Contact name |
Emery H. Bresnick |
| E-mail(s) |
ehbresni@facstaff.wisc.edu
|
| Phone |
608-265-6446
|
| Fax |
608-262-1257
|
| URL |
http://www.wisc.edu/molpharm/faculty/bresnick.html
|
| Organization name |
University of Wisconsin-Madison
|
| Department |
Pharmacology
|
| Lab |
Bresnick Lab
|
| Street address |
1300 University Avenue 383 MSC
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53706 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL9115 |
Illumina Genome Analyzer II (Homo sapiens) |
|
| Samples (3) |
|
| This SubSeries is part of SuperSeries: |
| GSE18829 |
Discovering Hematopoietic Mechanisms Through Genome-Wide Analysis of GATA Factor Chromatin Occupancy |
|
| Relations |
| SRA |
SRP001556 |
