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Series GSE18868 Query DataSets for GSE18868
Status Public on Nov 25, 2009
Title Genome-wide maps of GATA factor occupancy in K562 cells
Project ENCODE
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary GATA factors interact with simple DNA motifs (WGATAR) to regulate critical processes, including hematopoiesis, but very few WGATAR motifs are occupied in genomes. Given the rudimentary knowledge of mechanisms underlying this restriction, and how GATA factors establish genetic networks, we used ChIP-seq to define GATA-1 and GATA-2 occupancy genome-wide in erythroid cells. Coupled with genetic complementation analysis and transcriptional profiling, these studies revealed a rich collection of targets containing a characteristic binding motif of greater complexity than WGATAR. GATA factors occupied loci encoding multiple components of the Scl/TAL1 complex, a master regulator of hematopoiesis and leukemogenic target. Mechanistic analyses provided evidence for cross-regulatory and autoregulatory interactions among components of this complex, including GATA-2 induction of the hematopoietic corepressor ETO-2 and an ETO-2 negative autoregulatory loop. These results establish fundamental principles underlying GATA factor mechanisms in chromatin and illustrate a complex network of considerable importance for the control of hematopoiesis.

For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf
 
Overall design Examination of GATA1 and GATA2 occpancy in K562 cells
Web link http://www.ncbi.nlm.nih.gov/geo/info/ENCODE.html
 
Contributor(s) Fujiwara T, O'Geen H, Keles S, Blahnik K, Linnemann AK, Kang Y, Choi K, Farnham PJ, Bresnick EH
Citation(s) 19941826
BioProject PRJNA63447
Submission date Nov 03, 2009
Last update date May 15, 2019
Contact name Emery H. Bresnick
E-mail(s) ehbresni@facstaff.wisc.edu
Phone 608-265-6446
Fax 608-262-1257
URL http://www.wisc.edu/molpharm/faculty/bresnick.html
Organization name University of Wisconsin-Madison
Department Pharmacology
Lab Bresnick Lab
Street address 1300 University Avenue 383 MSC
City Madison
State/province WI
ZIP/Postal code 53706
Country USA
 
Platforms (1)
GPL9115 Illumina Genome Analyzer II (Homo sapiens)
Samples (3)
GSM467647 K562_GATA1_ChIP
GSM467648 K562_GATA2_ChIP
GSM467649 K562_Input
This SubSeries is part of SuperSeries:
GSE18829 Discovering Hematopoietic Mechanisms Through Genome-Wide Analysis of GATA Factor Chromatin Occupancy
Relations
SRA SRP001556

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE18868_RAW.tar 1.5 Gb (http)(custom) TAR (of BED, TXT)
SRA Run SelectorHelp
Processed data provided as supplementary file
Raw data are available in SRA

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