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| Status |
Public on Dec 17, 2021 |
| Title |
Single-cell RNA sequencing of repopulated microglia at day 3 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To probe potential molecular mechanisms underlying the differences between wild-type and Hk2-cKO microglia during repopulation, we performed the single-cell RNA sequencing (scRNA-seq) of repopulated microglia at Day 3. Microglia were isolated by FACS as CD11b high CD45 low cells from normal brains and Hk2-cKO brains.
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| Overall design |
Hk2 fl/fl and Hk2 cKO repopulated microglia at day3 were isolated by FACS as CD11b high CD45 low cells.
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| Contributor(s) |
Gao Z, Hu Y, Cao K |
| Citation missing |
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| BioProject |
PRJNA773981 |
| |
| Submission date |
Nov 11, 2021 |
| Last update date |
Dec 19, 2021 |
| Contact name |
weiying wu |
| E-mail(s) |
wuweiying@zju.edu.cn
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| Phone |
19883131534
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| Organization name |
Zhejiang University
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| Street address |
866 Yuhangtang Rd
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| City |
Hangzhou |
| State/province |
Zhejiang |
| ZIP/Postal code |
310027 |
| Country |
China |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (2) |
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