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| Status |
Public on Aug 02, 2022 |
| Title |
Wound healing in aged skin exhibits systems-level alterations in cellular composition and cell-cell communication |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Delayed and often impaired wound healing in the elderly presents major medical, social, and economic challenges. A systematic understanding of the cellular and molecular changes that shape complex cell-cell communications in aged skin wounds is lacking. Here we use single-cell RNA sequencing to define baseline differences across epithelial/fibroblast/immune cell types in young and aged skin during homeostasis and identify major changes in their subset compositions, kinetics, and molecular profiles during wound healing. Our data uncover a more pronounced inflammatory phenotype in aged skin wounds, featuring more neutrophils, a previously unknown inflammatory/glycolytic Arg1Hi macrophage subset, and fewer dendritic cells, compared to young counterparts. Generalizing our computational tool CellChat to compare signaling changes in young vs. aged skin wounds, we find specific alterations in Arg1Hi macrophage-fibroblast signaling interactions and the overall cell-cell communication networks. Finally, our systems-level and experimental analyses uncover dysregulated growth factor, chemokine, and cytokine pathways (e.g., IL-1, Ccl19-Ccr7) in aged skin wounds. Our study exposes numerous cellular and molecular targets for future functional interrogation.
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| Overall design |
Droplet-based single cell RNA-seq experiments were performed to identify molecular differences between young (7-week-old) and aged (88-week-old) skin from unwounded, 4-, and 7-day post-wounding skin.
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| Contributor(s) |
Dai X, Vu R, Jin S, Sun P |
| Citation(s) |
35926463 |
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| Submission date |
Nov 08, 2021 |
| Last update date |
Nov 01, 2022 |
| Contact name |
Remy Vu |
| E-mail(s) |
phuongnv@uci.edu
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| Organization name |
University of California, Irvine
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| Street address |
D288 Med Sci I Health Sciences Rd
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| City |
Irvine |
| ZIP/Postal code |
92697 |
| Country |
USA |
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| Platforms (2) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (11)
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| Relations |
| BioProject |
PRJNA778785 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE188432_aged_v3_batch1_barcodes.tsv.gz |
76.5 Kb |
(ftp)(http) |
TSV |
| GSE188432_aged_v3_batch1_features.tsv.gz |
272.8 Kb |
(ftp)(http) |
TSV |
| GSE188432_aged_v3_batch1_matrix.mtx.gz |
98.4 Mb |
(ftp)(http) |
MTX |
| GSE188432_young+aged_v3_batch2_barcodes.tsv.gz |
160.3 Kb |
(ftp)(http) |
TSV |
| GSE188432_young+aged_v3_batch2_features.tsv.gz |
272.8 Kb |
(ftp)(http) |
TSV |
| GSE188432_young+aged_v3_batch2_matrix.mtx.gz |
234.2 Mb |
(ftp)(http) |
MTX |
| GSE188432_young_v2_barcodes.tsv.gz |
151.8 Kb |
(ftp)(http) |
TSV |
| GSE188432_young_v2_genes.tsv.gz |
212.7 Kb |
(ftp)(http) |
TSV |
| GSE188432_young_v2_matrix.mtx.gz |
224.9 Mb |
(ftp)(http) |
MTX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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