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Series GSE18812 Query DataSets for GSE18812
Status Public on Oct 30, 2009
Title Comparative genome analysis of exosomal mRNA derived from Tspan8-overexpressing and Tspan8-negative rat pancreas tumors
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary We recently described that tumor-derived exosomes containing the tetraspanin Tspan8 induce angiogenesis. However, not much is known about exosome-endothelial cell interactions and there are no clues on the role of tetraspanins, which are a constitutive component of exosomes. We used the stably Tspan8 expressing rat adenocarcinoma cell line AS-Tspan8, to explore how exosomal Tspan8 supports angiogenesis. Tspan8 contributed to a selective recruitment of proteins and mRNA into exosomes. A genome-wide microarray analysis revealed that both, AS- and AS-Tspan8-exosomes contain about 1500 transcripts, from which 285 are over 3 fold enriched in AS-Tspan8-exosomes. We confirmed high levels mRNA of transcription factors MycN and Spi-C, elongation factor B(SIII), TCEB-1, fibroblast growth factor binding protein 1 (FGFBP1) and growth and differentiation factor-3 (GDF3) in AS-Tspan8-exosomes compared to AS-exosomes and to the parental cells by RT-PCR. VEGF mRNA was not enriched in AS-Tspan8 cells or exosomes. Expression of CCL20, prothrombin, bone morphogenetic protein-2, several Ras and MAPK family members and a phosphatase2A subunit was below the detection level in exosomes.
 
Overall design The array consists of 12 samples. Samples 1-6 represent total RNA from non-treated endothelial cells (1,2) cells co-cultured with Tspan8-negative exosomes (3,4) and cells co-cultured with Tspan8-positive exosomes (5,6). Samples 7 and 8 contain total RNA isolated from cells, from which exosomes are derived. Total RNA from Tspan8-negative exosomes represets samples 9,10; and total RNA from Tspan8-positive exosomes represents samples 11,12.
 
Contributor(s) Nazarenko I, Baumann A, Hellwig A, Zöller M
Citation(s) 20124479
Submission date Oct 29, 2009
Last update date Jul 29, 2014
Contact name Irina Nazarenko
E-mail(s) irina.nazarenko@kit.edu
Phone +49-1793237603
Organization name Karlsruhe Insitute of Technology (KIT)
Department Institute of Toxicology and Genetics
Street address Hermann-von-Helmholtz-Platz 1
City Eggenstein-Leopoldshafen
ZIP/Postal code 76344
Country Germany
 
Platforms (1)
GPL6101 Illumina ratRef-12 v1.0 expression beadchip
Samples (12)
GSM466551 EC_1
GSM466552 EC_2
GSM466553 ECmitAS_Exo_1
Relations
BioProject PRJNA121233

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE18812_Non-normalized.txt.gz 1.8 Mb (ftp)(http) TXT
GSE18812_RAW.tar 2.8 Mb (http)(custom) TAR
Processed data included within Sample table

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