|
| Status |
Public on Feb 20, 2010 |
| Title |
In depth sequencing of the siRNAs associated with peach latent mosaic viroid infection |
| Organisms |
Prunus persica; Prunus persica var. nucipersica |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
Using a high-throughput sequencing approach we quantitatively analyzed the content of viroid-derived siRNAs of an infected tree. Our results show that the entire PLMVd genome is found in the siRNA population. Also both polarities are susceptible to be targeted by the RNAi machinery but specific regions for each polarity are over represented. Those regions, that are not the same for each polarity, do not necessarily correlate with double stranded regions that could be substrate for Dicer-like enzymes. Finally the analysis of the first 5’ nucleotide revealed a bias toward a C or a U in viroid-derived siRNAs, indicating that at least AGO5 and AGO1 can recruit these small RNAs.
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| |
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| Overall design |
Analysis of siRNAs population from RNA sample isolated from a viroid-infected tree
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| |
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| Contributor(s) |
Bolduc F, Hoareau C, St-Pierre P, Perreault J |
| Citation(s) |
20158907 |
| |
| Submission date |
Oct 27, 2009 |
| Last update date |
May 15, 2019 |
| Contact name |
Jean-Pierre Perreault |
| E-mail(s) |
jean-pierre.perreault@usherbrooke.ca
|
| Phone |
819-564-5310
|
| Fax |
819-564-5340
|
| Organization name |
Université de Sherbrooke
|
| Department |
Biochimie
|
| Street address |
3001, 12ième avenue Nord
|
| City |
Sherbrooke |
| State/province |
Québec |
| ZIP/Postal code |
J1H5N4 |
| Country |
Canada |
| |
|
| Platforms (2) |
| GPL9487 |
Illumina Genome Analyzer (Prunus persica) |
| GPL9488 |
Illumina Genome Analyzer (Prunus persica var. nucipersica) |
|
| Samples (2) |
|
| Relations |
| SRA |
SRP001834 |
| BioProject |
PRJNA121611 |
