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| Status |
Public on May 01, 2022 |
| Title |
OCA-T1 and OCA-T2 are coactivators of POU2F3 in the tuft cell lineage |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
ChIP-Seq of C11orf53(OCA-T), COLCA2 and POU2F3(OCT11) in tuft cell lung cancer cell lines. Gene expression changes upon CRISPR knockout of different genes in tuft cell lung cancer cell lines and upon over-expression of C11orf53/POU2F3 alone or in combo in YT330 (a murine Notch high small cell lung cancer cell line)
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| Overall design |
ChIP-Seq in NCIH211, NCIH526, CORL311, and NCIH1048 cell lines with endeneous and lentivirally over-expressed construct. Gene expressoin changes in above mentioned cells and YT330 with CRISPR knockout or lentivirally over-expression. Single cell RNA-seq of wild-type and knockout mice.
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| Contributor(s) |
Wu XS, Preall JB, Vakoc CR |
| Citation(s) |
35576971 |
| |
| Submission date |
Oct 26, 2021 |
| Last update date |
Jul 08, 2022 |
| Contact name |
Xiaoli Sky Wu |
| Organization name |
Cold Spring Harbor Laboratory
|
| Street address |
1 Bungtown Road
|
| City |
Cold Spring Harbor |
| State/province |
New York |
| ZIP/Postal code |
11724 |
| Country |
USA |
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| Platforms (2) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (79)
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| Relations |
| BioProject |
PRJNA774795 |
| SRA |
SRP343255 |
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