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| Status |
Public on Mar 31, 2022 |
| Title |
14-3-3 protein constrains insulin secretion in pancreatic beta-cells by regulating mitochondrial function |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Members of the 14-3-3 protein family are often assumed to have redundant, over-lapping roles due to their high sequence homology and ubiquitous expression. To better understand the role of 14-3-3 in beta-cells, we generated beta-cell-specific 14-3-3 knockout (14-3-3KO) mice, 14-3-3KO mice displayed potentiated insulin secretion due to enhanced mitochondrial function and ATP synthesis.
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| Overall design |
Islets isolated from WT and beta14-3-3 KO mice at 12-14 weeks were dispersed after an overnight recovery. Dead cells were removed by using a dead cell removal kit and MS columns (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany). Single-cell RNA-seq libraries were made with the 10x Genomics Chromium Next GEM Single Cell 3' Library Kit (v3.1) and sequenced using an Illumina NovaSeq6000 (100bp x2, 50k reads/cell). Approximately 9600 cells were loaded per sample with an anticipated recovery of 6000 cells.
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| Web link |
https://pubmed.ncbi.nlm.nih.gov/35298439/
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| Contributor(s) |
Mugabo Y, Zhao C, Tan JJ, Ghosh A, Mugabo Y, Campbell SA, Fadzeyeva E, Paré F, Pan SS, Galipeau M, Ast J, Broichhagen J, Hodson DJ, Mulvihill EE, Petropoulos S, Lim GE |
| Citation(s) |
35298439 |
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| Submission date |
Oct 25, 2021 |
| Last update date |
Apr 02, 2022 |
| Contact name |
Cheng Zhao |
| E-mail(s) |
zhaocheng3326@gmail.com
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| Organization name |
Karolinska institute
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| Department |
Clinical Science
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| Lab |
Petropoulos/Lanner lab
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| Street address |
H9 Klinisk vetenskap,171 77 Stockholm
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| City |
Stockholm |
| State/province |
Stockholm |
| ZIP/Postal code |
171 77 |
| Country |
Sweden |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA774293 |
| SRA |
SRP343050 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE186529_RAW.tar |
361.2 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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