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| Status |
Public on Jan 19, 2022 |
| Title |
Effects of DNA topoisomerase inhibitor acriflavine on endothelial gene expression under hypoxic conditions |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
RNA-Seq of human endothelial cells treated with acriflavine (ACF) with or without hypoxia revealed massive changes on gene expression. These were non-randomly and potentially due to DNA topoisomerase inhibition rather than HIF inhibition. Surprisingly, in contrast to protein-coding genes, RNA-Seq yielded that an exceeding number of lncRNAs is upregulated.
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| |
|
| Overall design |
Examination of acriflavine treatment in HUVECs under hypoxia (16h, 1% O2)
|
| |
|
| Contributor(s) |
Seredinski S, Günther S, Brandes RP, Leisegang MS |
| Citation(s) |
35228897, 36700983 |
| |
| Submission date |
Oct 21, 2021 |
| Last update date |
Feb 07, 2023 |
| Contact name |
Stefan Günther |
| E-mail(s) |
stefan.guenther@mpi-bn.mpg.de
|
| Organization name |
MPI for heart and lung research
|
| Street address |
ludwigtr. 43
|
| City |
bad nauheim |
| ZIP/Postal code |
61231 |
| Country |
Germany |
| |
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| Platforms (1) |
|
| Samples (6)
|
| GSM5643366 |
Hypoxia_Acriflavine_HUVEC_1 [Hyp_ACF_1] |
| GSM5643367 |
Hypoxia_Acriflavine_HUVEC_2 [Hyp_ACF_2] |
| GSM5643368 |
Hypoxia_Acriflavine_HUVEC_3 [Hyp_ACF_3] |
|
| Relations |
| BioProject |
PRJNA773246 |
| SRA |
SRP342506 |
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