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| Status |
Public on Jun 23, 2010 |
| Title |
Eicosapentaenoic acid improves metabolic switching in human myotubes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Metabolically healthy skeletal muscle is characterized by the ability to switch easily between glucose and fat oxidation, whereas loss of this ability seems to be related to insulin resistance. The aim of this study was to investigate whether different fatty acids (FAs) and the LXR ligand T0901317 affected metabolic switching in human skeletal muscle cells (myotubes). Pretreatment of myotubes with eicosapentaenoic acid (EPA) increased suppressibility, the ability of glucose to suppress FA oxidation, and metabolic flexibility, the ability to increase FA oxidation when changing from “fed” to “fasted” state. Adaptability, the capacity to increase FA oxidation with increasing FA availability, was increased after pretreatment with EPA, linoleic acid (LA) and palmitic acid (PA). T0901317 counteracted the effect of EPA on suppressibility and adaptability, but did not affect these parameters alone. EPA itself accumulated less, however, EPA, LA, OA and T0901317 increased the number of lipid droplets (LDs) in myotubes, whereas LD size and mitochondria amount were independent of pretreatment. Microarray analysis showed that EPA regulated more genes than the other FAs. Some pathways involved in carbohydrate metabolism were induced only by EPA. The present study suggests a possible favorable effect of EPA on skeletal muscle metabolic switching and glucose utilization.
Keywords: Analysis of target gene regulation by using microarrays.
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| Overall design |
Primary human myotubes, derived from 3 healthy, female donors, were preincubated with different fatty acids (oleic acid [OA], palmitic acid [PA], eicosapentaenoic acid [EPA] or linoleic acid [LA], each at 100 µM) or bovine serum albumin [BSA] (40 µM) for 24 h.
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| Contributor(s) |
Hessvik NP, Bakke SS, Fredriksson K, Boekschoten MV, Fjorkenstad A, Koster G, Hesselink MK, Kersten S, Kase ET, Rustan AC, Thoresen H |
| Citation(s) |
20363834, 22796147, 24591600, 30496314 |
| Submission date |
Oct 15, 2009 |
| Last update date |
Dec 27, 2018 |
| Contact name |
Guido Hooiveld |
| E-mail(s) |
guido.hooiveld@wur.nl
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| Organization name |
Wageningen University
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| Department |
Div. Human Nutrition & Health
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| Lab |
Nutrition, Metabolism & Genomics Group
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| Street address |
HELIX, Stippeneng 4
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| City |
Wageningen |
| ZIP/Postal code |
NL-6708WE |
| Country |
Netherlands |
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| Platforms (1) |
| GPL7020 |
NuGO array (human) NuGO_Hs1a520180 |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA120303 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE18589_RAW.tar |
36.8 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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