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| Status |
Public on Aug 11, 2023 |
| Title |
Single-Cell RNA-Sequencing reveals cellular heterogeneity and traiectories of lineage specification of human primary HCC tumorspheres |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We report the application of Single-Cell RNA-Sequencing technology for high-throughput profiling in human primary HCC tumorspheres. By obtaining human primary HCC cells isolated from tumor tissues of PDX mice, we proceeded sphere-forming culture and then performed Single-Cell RNA-Sequencing of human primary HCC cells. The scRNA-Seq libraries were prepared with Single Cell 3′ Reagent Kit v2 (10×Genomics) following the user guide provided. We found that SCARB2 positive cells accounted about 11.3% of total tumorspheres and were enriched with MYC target genes. We conclude that Single-Cell RNA-Sequencing would expedite genetic network analyses and permit the dissection of complex biologic functions.
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| Overall design |
one sample from human primary HCC tumorspheres
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| Contributor(s) |
Li K, Jiang J, Wang F, Gao Y, Xue S |
| Citation(s) |
37739936 |
| |
| Submission date |
Oct 13, 2021 |
| Last update date |
Oct 03, 2023 |
| Contact name |
Yang Gao |
| E-mail(s) |
yxgyang@126.com
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| Phone |
18238837867
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| Organization name |
Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College
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| Street address |
Dongcheng District, Tiantan Xili
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| City |
Beijing |
| ZIP/Postal code |
100050 |
| Country |
China |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (1) |
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| This SubSeries is part of SuperSeries: |
| GSE185844 |
Scavenger Receptor SCARB2 Drives Hepatic Carcinoma Initiation by Enhancing MYC Transcriptional Activity and Supporting Cancer Stem Cell Traits. |
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| Relations |
| BioProject |
PRJNA770981 |
| SRA |
SRP341221 |
