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| Status |
Public on Oct 09, 2009 |
| Title |
Expression profiling of hypoxic HepG2 hepatoma, U87 glioma, and MDA-MB231 breast cancer cells: time course |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Analysis of expression changes of cultured HepG2 hepatoma, U87 glioma, and MDA-MB231 breast cancer cells subjected to hypoxia (0.5% O2) for 0, 4, 8, 12 hours . Results provide insight to cell type-specific response to hypoxia.
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| Overall design |
HepG2 hepatoma, U87 glioma, and MDA-MB231 breast cancer cells were collected under normoxic conditions (~19% O2, 0 hours) and after 4, 8 and 12 hours of hypoxia treatment (0.5% O2). For each cell line, three replicates of total RNA at each time point were prepared using Trizol and submitted to the DFCI Microarray Core for labeling, hybridization to Affymetrix HG-U133Plus2 oligonucleotide arrays and image scanning.
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| Contributor(s) |
Xia X, Kung AL |
| Citation(s) |
19828020 |
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| Submission date |
Oct 09, 2009 |
| Last update date |
Mar 21, 2012 |
| Contact name |
Xiaobo Xia |
| E-mail(s) |
Xiaobo_Xia@dfci.harvard.edu
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| Organization name |
Dana-Farber Cancer Institute
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| Department |
Pediatric Oncology
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| Lab |
Dr. Andrew Kung
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| Street address |
44 Binney St, Mayer 649
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (1) |
| GPL9419 |
Affymetrix GeneChip Human Genome U133 Plus 2.0 Array [CDF: Hs133P_Hs_REFSEQ_10] |
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| Samples (36)
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| GSM460669 |
HepG2 hypoxia (0.5%O2) treatment 0h, biological rep1 |
| GSM460670 |
HepG2 hypoxia (0.5%O2) treatment 0h, biological rep2 |
| GSM460671 |
HepG2 hypoxia (0.5%O2) treatment 0h, biological rep3 |
| GSM460672 |
HepG2 hypoxia (0.5%O2) treatment 4h, biological rep1 |
| GSM460673 |
HepG2 hypoxia (0.5%O2) treatment 4h, biological rep2 |
| GSM460674 |
HepG2 hypoxia (0.5%O2) treatment 4h, biological rep3 |
| GSM460675 |
HepG2 hypoxia (0.5%O2) treatment 8h, biological rep1 |
| GSM460676 |
HepG2 hypoxia (0.5%O2) treatment 8h, biological rep2 |
| GSM460677 |
HepG2 hypoxia (0.5%O2) treatment 8h, biological rep3 |
| GSM460678 |
HepG2 hypoxia (0.5%O2) treatment 12h, biological rep1 |
| GSM460679 |
HepG2 hypoxia (0.5%O2) treatment 12h, biological rep2 |
| GSM460680 |
HepG2 hypoxia (0.5%O2) treatment 12h, biological rep3 |
| GSM460681 |
U87 hypoxia (0.5%O2) treatment 0h, biological rep1 |
| GSM460682 |
U87 hypoxia (0.5%O2) treatment 0h, biological rep2 |
| GSM460683 |
U87 hypoxia (0.5%O2) treatment 0h, biological rep3 |
| GSM460684 |
U87 hypoxia (0.5%O2) treatment 4h, biological rep1 |
| GSM460685 |
U87 hypoxia (0.5%O2) treatment 4h, biological rep2 |
| GSM460686 |
U87 hypoxia (0.5%O2) treatment 4h, biological rep3 |
| GSM460687 |
U87 hypoxia (0.5%O2) treatment 8h, biological rep1 |
| GSM460688 |
U87 hypoxia (0.5%O2) treatment 8h, biological rep2 |
| GSM460689 |
U87 hypoxia (0.5%O2) treatment 8h, biological rep3 |
| GSM460690 |
U87 hypoxia (0.5%O2) treatment 12h, biological rep1 |
| GSM460691 |
U87 hypoxia (0.5%O2) treatment 12h, biological rep2 |
| GSM460692 |
U87 hypoxia (0.5%O2) treatment 12h, biological rep3 |
| GSM460693 |
MB231 hypoxia (0.5%O2) treatment 0h, biological rep1 |
| GSM460694 |
MB231 hypoxia (0.5%O2) treatment 0h, biological rep2 |
| GSM460695 |
MB231 hypoxia (0.5%O2) treatment 0h, biological rep3 |
| GSM460696 |
MB231 hypoxia (0.5%O2) treatment 4h, biological rep1 |
| GSM460697 |
MB231 hypoxia (0.5%O2) treatment 4h, biological rep2 |
| GSM460698 |
MB231 hypoxia (0.5%O2) treatment 4h, biological rep3 |
| GSM460699 |
MB231 hypoxia (0.5%O2) treatment 8h, biological rep1 |
| GSM460700 |
MB231 hypoxia (0.5%O2) treatment 8h, biological rep2 |
| GSM460701 |
MB231 hypoxia (0.5%O2) treatment 8h, biological rep3 |
| GSM460702 |
MB231 hypoxia (0.5%O2) treatment 12h, biological rep1 |
| GSM460703 |
MB231 hypoxia (0.5%O2) treatment 12h, biological rep2 |
| GSM460704 |
MB231 hypoxia (0.5%O2) treatment 12h, biological rep3 |
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| This SubSeries is part of SuperSeries: |
| GSE18505 |
HepG2 hepatoma and U87 glioma cells: transcriptomic and genomic analyses |
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| Relations |
| BioProject |
PRJNA123717 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE18494_Hypoxia_expression_summary.txt.gz |
1.6 Mb |
(ftp)(http) |
TXT |
| GSE18494_RAW.tar |
312.7 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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