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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Nov 22, 2021 |
| Title |
Deleting DNMT3A in CAR T cells prevents exhaustion and 1 enhances antitumor activity |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
Whole-genome bisulfite sequencing data generated from WT and DNMT3A KO or IL-10 KO CAR T cells. These data were generate at early (week 1) and later (week 4) time points during in vitro serial stimulation of the CAR T cells, or after infusion into tumor bearing mice. These data document the DNA methylation changes that are coupled to the functional decline in CAR T cells during their sustained stimulation, and the causal nature of DNMT3A-specific methylation in mediating the functional impairment of the CAR T cells.
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| Overall design |
Total human T cells were transduced with mock or KO specific guide RNAs complexed with CAS. The gene-edited T cells were then transduce with a vector for expressing a chimeric antigen receptor (CAR). The WT and KO CAR T cells were then cultured in the presence of tumor cells that express the cognate antigen to stimulate the CAR-T cells. DNA was extracted from the stimulated CAR T cells at the specified time points and subjected to whole-genome bisulfite sequencing.
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| Contributor(s) |
Prinzing B, Zebley CC, Petersen CT, Fan Y, Anido AA, Yi Z, Nguyen P, Houke H, Bell M, Haydar D, Brown C, Boi S, Alli S, Crawford JC, Riberdy J, Park J, Zhou S, Velasquez MP, DeRenzo C, Lazzarotto CR, Tsai SQ, Vogel P, Pruett-Miller SM, Langfitt D, Gottschalk S, Youngblood B, Krenciute G |
| Citation(s) |
34788079 |
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| Submission date |
Sep 21, 2021 |
| Last update date |
Nov 23, 2021 |
| Contact name |
yiping fan |
| E-mail(s) |
yiping.fan@stjude.org
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| Phone |
9015955716
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| Organization name |
st jude children's hospital
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| Street address |
262 Danny Thomas Place
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| City |
memphis |
| State/province |
tn |
| ZIP/Postal code |
38105 |
| Country |
USA |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (41)
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| Relations |
| BioProject |
PRJNA765208 |
| SRA |
SRP338189 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE184568_RAW.tar |
8.1 Gb |
(http)(custom) |
TAR (of BED) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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