|
| Status |
Public on Jan 17, 2022 |
| Title |
Transcriptome-wide map of m6A RNAs in A375 NC cells and shMETTL3 cells |
| Organism |
Homo sapiens |
| Experiment type |
Other
|
| Summary |
The newly discovered dynamic N6-methyladenosine (m6A) modification plays a critical role in gene expression from a post-transcriptional level. we profiled the transcriptome-wide m6A modification in mRNAs and lncRNAs in non-targeting control A375 cells(NC) and METTL3 knockdown A375 cells(shMETTL3). Methylated RNA immunoprecipitation sequencing results revealed that the RNA m6A modification is conserved. METTL3 knockdown altered the expression of RNAs m6A modified sites in A375 cells. Finally, we show that the transcriptome-wide m6A alterations occurring in mRNAs and lncRNAs following METTL3 knockdown suggest this process plays important regulatory roles during A375 growth. This study provides a framework for applying the m6A modification regulated by METTL3 to melanoma research.
|
| |
|
| Overall design |
Examination of m6A modification change and gene expression alternation in A375 NC and shMETTL3 cells
|
| |
|
| Contributor(s) |
Sun J, Yue Z, Wang Y |
| Citation(s) |
35117988 |
| |
| Submission date |
Sep 12, 2021 |
| Last update date |
Feb 08, 2022 |
| Contact name |
Zhanghui Yue |
| E-mail(s) |
yuezh2016@outlook.com
|
| Phone |
17502550495
|
| Organization name |
Hospital for Skin Diseases (Institute of Dermatology), Chinese Academy of Medical Sciences & Peking Union Medical College
|
| Street address |
12 Jiangwangmiao Street, Xuanwu Area
|
| City |
Nanjing |
| State/province |
Jiangsu |
| ZIP/Postal code |
210042 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA762550 |
| SRA |
SRP336724 |
Less...
More...