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Series GSE18344 Query DataSets for GSE18344
Status Public on Feb 06, 2010
Title The Transcriptome of Nrf2-Deficient Mice in Cigarette Smoke-Induced Emphysematous Changes
Organism Mus musculus
Experiment type Expression profiling by array
Summary Cigarette smoke (CS) imposes a strong oxidative burden on exposed tissues resulting in a severely disturbed oxidant/antioxidant balance, which in the context of chronic exposure is assumed to be a key contributor to CS-related diseases. Because of its emerging central role in orchestrating the general cellular antioxidant response, the pathway leading to the activation of the transcription factor Nrf2 has received mounting attention over the past decade in investigations aimed at elucidating CS-induced patho-physiological mechanisms. To comprehensively characterize the impact of Nrf2 in acute and sub-chronic smoking scenarios, Nrf2 knock-out mice and their wildtype ICR littermates were exposed to either ambient air (sham exposure) or to one of three doses of CS for up to 5 months with two post-exposure endpoints of 1 and 13 days. The lungs of the mice were monitored for transcriptomic changes on a genome-wide level.
 
Overall design 110 samples from 28 different groups are analyzed. For each group there are 4 replicates, besides two groups with only 3 replicates. Group parameteres are: genotype (WT, KN), treatment (sham, smoke), dosage of smoke treatment (low, medium, high), time of smoke treatment (1 day, 2 month, 5 month, 5 month + 1 day recovery, 5 month + 13 days recovery)
 
Contributor(s) Gebel S, Mueller T, Diehl S, Schüller J, Yamamoto M
Citation(s) 20133372
Submission date Sep 30, 2009
Last update date Feb 11, 2019
Contact name Stephan Gebel
E-mail stephan.gebel@pmintl.com
Organization name Philip Morris Research Laboratories
Street address Fuggerstr. 3
City Koeln
ZIP/Postal code 51149
Country Germany
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (110)
GSM457905 KN-sham-1 day-R1
GSM457906 KN-sham-1 day-R2
GSM457907 KN-sham-1 day-R3
Relations
BioProject PRJNA118151

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE18344_RAW.tar 404.5 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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