NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE183288 Query DataSets for GSE183288
Status Public on Oct 13, 2022
Title Single-cell dissection of obesity-exercise axis in adipose-muscle tissues
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We studied the opposing effects of exercise training and high-fat diet at single-cell resolution to reveal changes in cell type abundance, cell-type-specific gene expression/pathway/regulatory network changes, and changes in cell-cell communication both within and across tissues. We profiled scRNA-seq in 204,883 cells, grouped into 53 distinct cell sub-types/states across 22 major cell types, from subcutaneous white adipose tissue, visceral white adipose tissue, and skeletal muscle across 16 lean and 15 obese mice with both diet and exercise interventions. Changes in both cell proportion and transcriptional state were most strongly pronounced in adipose stem cells in fat, consistent with roles of adipogenesis in thermogenesis-vs-lipogenesis and hyperplasia-vs-hypertrophy in obesity. For immune cells, exercise training decreases obesity-associated inflammatory tissue-resident cell populations, including myeloid and regulatory T cells, and promotes beige-cell-inducing populations, including NKT cells. These changes clustered in common pathways across tissues for both exercise and obesity, including extracellular matrix remodelling and circadian rhythm in mesenchymal stem cells and cell activation/migration in immune cells.
 
Overall design Single-cell mRNA profiles of three metabolic tissues from 6-week old C57BL/6JN mice with 6-week high-fat diet and 3-week exercise training interventions
Web link http://scmetab.mit.edu
 
Contributor(s) Yang J, Vamvini M, Nigro P, Goodyear L, Kellis M
Citation(s) 36198295
Submission date Sep 02, 2021
Last update date Mar 02, 2026
Contact name Jiekun Yang
E-mail(s) jackie.yang@rutgers.edu
Organization name Rutgers University
Street address 145 Bevier Rd
City Piscataway
State/province NJ
ZIP/Postal code 08854
Country USA
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (42)
GSM5554964 Single-cell sedentary standard (chow) diet rep1 [SC1.scWAT]
GSM5554965 Single-cell sedentary standard (chow) diet rep2 [SC2.scWAT]
GSM5554966 Single-cell sedentary standard (chow) diet rep3 [SC3.scWAT]
This SubSeries is part of SuperSeries:
GSE183290 Dissection of obesity-exercise axis in adipose-muscle tissues
Relations
BioProject PRJNA759895
SRA SRP335420

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE183288_Single_cell_atlas.qs.gz 3.1 Gb (ftp)(http) QS
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap