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Series GSE18319 Query DataSets for GSE18319
Status Public on Feb 20, 2010
Title Gene expression signatures for mESC treated by RNAi strategy on down regulation of gene Mtf2
Organism Mus musculus
Experiment type Expression profiling by array
Summary To further explore the function of Mtf2, the homolog of Drosophila Polycomb group gene PCL, we have employed the mouse whole genome microarray expression profiling as a discovery platform to identify genes mediated by Mtf2. Oligos of 2 different RNAi target sites on gene Mtf2 were cloned into RNAi Vector pSuper and transfected individually into Mouse Embryonic Stem Cell line E14. Empty Vector pSuper and plasmid containing RNAi target against gene Luciferase (Firefly) were performed in the same way as negative controls. The down regulation effect of gene Mtf2 was quantified by real-time PCR in the same RNA samples subjected to microarray analysis.
 
Overall design mESC was transfected by RNAi plasmids with resistence of puromycin and selected by Puromycin(1mg/ml) for 2 days. 4 independent experiments were performed for vector pSuper, plasimd pSuper-Luci-RNAi, pSuper-Mtf2-RNAi-1, pSuper-Mtf2-RNAi-3.
 
Contributor(s) Xie D
Citation(s) 20219939
Submission date Sep 29, 2009
Last update date May 10, 2018
Contact name Dan Xie
E-mail(s) danxie2@uiuc.edu
Organization name University of Illinois at Urbana and Champaign
Department Bioengineering
Street address 3215 Digital Computer Lab, MC-278, 1304 W. Springfield Ave
City Urbana
State/province IL
ZIP/Postal code 61801
Country USA
 
Platforms (1)
GPL4134 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version)
Samples (4)
GSM457323 pSuper_2days
GSM457324 Luci_RNAi_2days
GSM457325 Mtf2_RNAi_1_2days
Relations
BioProject PRJNA117971

Download family Format
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Supplementary file Size Download File type/resource
GSE18319_RAW.tar 23.5 Mb (http)(custom) TAR (of GPR)
SRA Run SelectorHelp
Raw data provided as supplementary file
Processed data included within Sample table

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