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| Status |
Public on Jan 07, 2022 |
| Title |
POLIE suppresses telomerase-mediated telomere G-strand extension and helps ensure proper telomere C-strand synthesis in trypanosomes |
| Organism |
Trypanosoma brucei brucei |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
T. brucei regularly switches its major surface antigen, VSG, to evade the mammalian host immune response. VSGs are expressed from subtelomeric loci in a strictly monoallelic manner. We identify POLIE as an intrinsic component of the T. brucei telomere complex and examined the effect of POLIE depletion on VSG expression regulation by RNAseq.
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| Overall design |
Tet-inducible POLIE RNAi strains were established. Total RNA was isolated from POLIE RNAi cells before or 30 hr after the induction of POLIE RNAi. Two biological replicates were included. RNA samples were sent to Novogene for subsequent experiments. Poly(A) RNA was purified for generating cDNA library and paired-end sequencing was done using Illumina. Gene expression profiles were compared between the induced and uninduced cells by differential gene expression analysis.
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| Web link |
https://academic.oup.com/nar/article/50/4/2036/6513566#supplementary-data
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| Contributor(s) |
Rabbani MA, Tonini ML, Afrin M, Li B |
| Citation(s) |
35061898 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 AI066095 |
Mechanisms of RAP1 functions in monoallelic VSG expression in Trypanosoma brucei |
CLEVELAND STATE UNIVERSITY |
Bibo Li |
| R01 AI127562 |
Molecular dynamics of genome and epigenome integrity in Trypanosoma brucei |
RUTGERS, THE STATE UNIVERSITY OF NEW JERSEY--RBHS-NEW JERSEY MEDICAL SCHOOL |
Hee-Sook Kim |
| S10 OD025252 |
A Digital Laser Scanner Biomolecular Imaging System: Amersham Typhoon 5 |
CLEVELAND STATE UNIVERSITY |
Bibo Li |
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| |
| Submission date |
Aug 27, 2021 |
| Last update date |
Apr 08, 2022 |
| Contact name |
Bibo Li |
| E-mail(s) |
b.li37@csuohio.edu
|
| Phone |
216 6872444
|
| Organization name |
CLEVELAND STATE University
|
| Department |
Center for Gene Regulation in Health and Disease
|
| Street address |
2121 Euclid Avenue
|
| City |
CLEVELAND |
| State/province |
OH |
| ZIP/Postal code |
44115 |
| Country |
USA |
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| Platforms (1) |
| GPL27986 |
Illumina NovaSeq 6000 (Trypanosoma brucei brucei) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA758389 |
| SRA |
SRP334482 |
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