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| Status |
Public on Jun 15, 2022 |
| Title |
Tet2-mediated epigenetic programing of T follicular helper cell differentiation (Methyl-Seq) |
| Organism |
Mus musculus |
| Experiment type |
Methylation profiling by high throughput sequencing
|
| Summary |
Tet2-mediated demethylation is a key component of epigenetic programing that promotes lineage specific gene expression and contributes to cellular differentiation and function. While the differentiation of CD4+ T cell subsets has been studied extensively, the epigenetic programs that regulate these processes remain unclear. We report that Tet2 acts to restrict the differentiation of T follicular helper (Tfh) cells in CD4+ T cells responding to viral infection. Tet2-deficient CD4+ T cells preferentially differentiated into highly functional germinal center (GC) Tfh cells that provided enhanced help for B cell responses. Using genome-wide expression and methylation analyses combined with Foxo1 ChIPseq analysis, we found that Tet2 coordinates with multiple transcription factors, including Foxo1, to mediate the demethylation and expression of their target genes following activation.
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| Overall design |
Mouse antigen specific WT and Tet2KO CD4+ T cells (SMARTA TCR Transgenic) were sorted from spleens of non-infected mice (CD4+ T cell naïve samples) and LCMV Armstrong infected mice at 7 days post infection (effector Th1 and effector Tfh samples) for genome wide enzymatic methylation sequencing.
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| Contributor(s) |
Baessler AS, Wadsworth ME, Varley KE, Hale JS |
| Citation(s) |
35704571 |
| |
| Submission date |
Aug 27, 2021 |
| Last update date |
Jul 07, 2022 |
| Contact name |
Jeffrey Scott Hale |
| E-mail(s) |
scott.hale@path.utah.edu
|
| Phone |
801-587-1885
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| Organization name |
University of Utah
|
| Department |
Pathology
|
| Lab |
Hale Lab
|
| Street address |
15 North Medical Drive
|
| City |
Salt Lake City |
| State/province |
Utah |
| ZIP/Postal code |
84112 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (18)
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| This SubSeries is part of SuperSeries: |
| GSE183317 |
Tet2-mediated epigenetic programing of T follicular helper cell differentiation |
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| Relations |
| BioProject |
PRJNA758390 |
| SRA |
SRP334481 |
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