|
| Status |
Public on Jul 28, 2010 |
| Title |
IGH analysis in pro-B cells with H3K4me3, H3K4me2 and H3K9ac |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
|
| Summary |
STAT5 and IL-7 signaling are thought to control B-lymphopoiesis by regulating key transcription factor genes and activating VH gene segments at the Igh locus. Using conditional mutagenesis, we demonstrate that transgenic Bcl2 expression rescued the development of Stat5-deleted pro-B cells by compensating for the loss of Mcl-1. Ebf1 and Pax5 expression as well as VH recombination were normal in Bcl2-rescued pro-B cells lacking STAT5 or IL-7Ra. In agreement with this finding, STAT5-expressing pro-B cells contained little or no active chromatin at most VH genes. In contrast, Igk rearrangements were increased in STAT5- or IL-7Ra-deficient pro-B cells, consistent with direct binding of STAT5 to the intronic iEk enhancer in wild-type pro-B cells. Hence, STAT5 and IL-7 signaling control cell survival and suppress premature Igk recombination in early B-lymphopoiesis.
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| |
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| Overall design |
comparison of wt vs Rag2-/- pro-B cells
|
| |
|
| Contributor(s) |
McManus S, Busslinger M |
| Citation(s) |
19946273 |
| |
| Submission date |
Sep 25, 2009 |
| Last update date |
Mar 21, 2012 |
| Contact name |
Meinrad Busslinger |
| E-mail(s) |
busslinger@imp.ac.at
|
| Phone |
00431-79730
|
| Organization name |
Instutute of Molecular Pathology
|
| Street address |
Dr. Bohrgasse 7
|
| City |
Vienna |
| ZIP/Postal code |
1030 |
| Country |
Austria |
| |
|
| Platforms (2) |
|
| Samples (9)
|
| GSM456492 |
GenAu MB BCELL v3.0 H3K4me2 Rag2-/- |
| GSM456493 |
GenAu MB BCELL v3.0 H3Kme3 Rag2-/- |
| GSM456494 |
GenAu MB BCELL v3.0 H3K9ac Rag2-/- |
|
| Relations |
| BioProject |
PRJNA117955 |
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