NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE182774 Query DataSets for GSE182774
Status Public on Nov 01, 2023
Title Single cell analysis of meningeal ectopic lymphoid tissue and secondary lymphoid tissues in a spontaneous EAE model
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Other
Summary Objective: To investigate how similar meningeal ectopic lymhpoid tissue (mELT) is to secondary lymphoid tissues and find unique features of mELT that could allow therapeutic targetting of mELT specifically. Methods: We utilized a spontaneous chronic experimental autoimmune encephalomyelitis (EAE) model of mice with mutant T and B cell receptors specific for myelin oligodendrocyte glycoprotein (MOG), which develop meningeal inflammatory infiltrates resembling those described in Multiple Sclerosis (MS). We identified differences in cell composition and gene expression between mELT and secondary lymphoid tissues (lumbar and inguinal lymph nodes and the spleen) using single cell sequencing.
 
Overall design single cell gene expression and Bcell receptor sequences of meningeal ectopic lymphoid tissue, blood, spleen, inguinal and lumbar lymph nodes in 3 Thx2D2 mice
 
Contributor(s) Lepennetier G, Lehmann-Horn K, Diddens J, Georgieva T, Friederich V
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Aug 25, 2021
Last update date Nov 01, 2023
Contact name Gildas Lepennetier
E-mail(s) gildas.lepennetier@tum.de
Organization name Klinikum Rechts der Isar
Department Neurology
Street address Ismaninger Straße 22
City Munich
ZIP/Postal code 81675
Country Germany
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (27)
GSM5536798 MUC25985
GSM5536799 MUC25986
GSM5536800 MUC25987
Relations
BioProject PRJNA757745
SRA SRP334107

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE182774_Feature_mouse_10x.csv.gz 342 b (ftp)(http) CSV
GSE182774_I18_barcodes.tsv.gz 136.2 Kb (ftp)(http) TSV
GSE182774_I18_features.tsv.gz 284.3 Kb (ftp)(http) TSV
GSE182774_I18_filtered_contig_annotations.csv.gz 417.2 Kb (ftp)(http) CSV
GSE182774_I18_matrix.mtx.gz 112.3 Mb (ftp)(http) MTX
GSE182774_J119_barcodes.tsv.gz 114.4 Kb (ftp)(http) TSV
GSE182774_J119_features.tsv.gz 284.3 Kb (ftp)(http) TSV
GSE182774_J119_filtered_contig_annotations.csv.gz 237.8 Kb (ftp)(http) CSV
GSE182774_J119_matrix.mtx.gz 87.3 Mb (ftp)(http) MTX
GSE182774_J9_barcodes.tsv.gz 135.1 Kb (ftp)(http) TSV
GSE182774_J9_features.tsv.gz 284.3 Kb (ftp)(http) TSV
GSE182774_J9_filtered_contig_annotations.csv.gz 392.2 Kb (ftp)(http) CSV
GSE182774_J9_matrix.mtx.gz 125.4 Mb (ftp)(http) MTX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap