|
| Status |
Public on Nov 01, 2023 |
| Title |
Single cell analysis of meningeal ectopic lymphoid tissue and secondary lymphoid tissues in a spontaneous EAE model |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
|
| Summary |
Objective: To investigate how similar meningeal ectopic lymhpoid tissue (mELT) is to secondary lymphoid tissues and find unique features of mELT that could allow therapeutic targetting of mELT specifically. Methods: We utilized a spontaneous chronic experimental autoimmune encephalomyelitis (EAE) model of mice with mutant T and B cell receptors specific for myelin oligodendrocyte glycoprotein (MOG), which develop meningeal inflammatory infiltrates resembling those described in Multiple Sclerosis (MS). We identified differences in cell composition and gene expression between mELT and secondary lymphoid tissues (lumbar and inguinal lymph nodes and the spleen) using single cell sequencing.
|
| |
|
| Overall design |
single cell gene expression and Bcell receptor sequences of meningeal ectopic lymphoid tissue, blood, spleen, inguinal and lumbar lymph nodes in 3 Thx2D2 mice
|
| |
|
| Contributor(s) |
Lepennetier G, Lehmann-Horn K, Diddens J, Georgieva T, Friederich V |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Aug 25, 2021 |
| Last update date |
Nov 01, 2023 |
| Contact name |
Gildas Lepennetier |
| E-mail(s) |
gildas.lepennetier@tum.de
|
| Organization name |
Klinikum Rechts der Isar
|
| Department |
Neurology
|
| Street address |
Ismaninger Straße 22
|
| City |
Munich |
| ZIP/Postal code |
81675 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (27)
|
|
| Relations |
| BioProject |
PRJNA757745 |
| SRA |
SRP334107 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE182774_Feature_mouse_10x.csv.gz |
342 b |
(ftp)(http) |
CSV |
| GSE182774_I18_barcodes.tsv.gz |
136.2 Kb |
(ftp)(http) |
TSV |
| GSE182774_I18_features.tsv.gz |
284.3 Kb |
(ftp)(http) |
TSV |
| GSE182774_I18_filtered_contig_annotations.csv.gz |
417.2 Kb |
(ftp)(http) |
CSV |
| GSE182774_I18_matrix.mtx.gz |
112.3 Mb |
(ftp)(http) |
MTX |
| GSE182774_J119_barcodes.tsv.gz |
114.4 Kb |
(ftp)(http) |
TSV |
| GSE182774_J119_features.tsv.gz |
284.3 Kb |
(ftp)(http) |
TSV |
| GSE182774_J119_filtered_contig_annotations.csv.gz |
237.8 Kb |
(ftp)(http) |
CSV |
| GSE182774_J119_matrix.mtx.gz |
87.3 Mb |
(ftp)(http) |
MTX |
| GSE182774_J9_barcodes.tsv.gz |
135.1 Kb |
(ftp)(http) |
TSV |
| GSE182774_J9_features.tsv.gz |
284.3 Kb |
(ftp)(http) |
TSV |
| GSE182774_J9_filtered_contig_annotations.csv.gz |
392.2 Kb |
(ftp)(http) |
CSV |
| GSE182774_J9_matrix.mtx.gz |
125.4 Mb |
(ftp)(http) |
MTX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
Less...
More...