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| Status |
Public on Mar 01, 2010 |
| Title |
Global gene expression analysis of transgenic, mannitol-producing, and salt-tolerant Arabidopsis thaliana indicates widespread changes in abiotic and biotic stress-related genes |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
Mannitol is a putative osmoprotectant contributing to salt tolerance in several species. Arabidopsis plants transformed with the mannose-6-phosphate reductase (M6PR) gene from celery were dramatically more salt tolerant (at 100 mM NaCl) as exhibited by reduced salt injury, less inhibition of vegetative growth, and increased seed production relative to the wild type (WT). When treated with 200 mM NaCl, transformants produced no seeds, but did bolt, and exhibited less chlorosis/necrosis and greater survival and dry weights than the WT. Without salt there were no M6PR effects on growth or phenotype, but expression levels of 2272 genes were altered. Many fewer differences (1039) were observed between M6PR and WT plants in the presence of salt, suggesting that M6PR pre-conditioned the plants to stress. Previous work suggested that mannitol is an osmoprotectant, but mannitol levels are invariably quite low, perhaps inadequate for osmoprotectant effects. In this study, transcriptome analysis reveals that the M6PR transgene activated the downstream abscisic acid (ABA) pathway by up-regulation of ABA receptor genes (PYL4, PYL5, and PYL6) and down-regulation of protein phosphatase 2C genes (ABI1 and ABI2). In the M6PR transgenic lines there were also increases in transcripts related to redox and cell wall-strengthening pathways. These data indicate that mannitol-enhanced stress tolerance is due at least in part to increased expression of a variety of stress-inducible genes.
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| Overall design |
Both M6PR transgenic and Col WT plants were grown in the growth chamber in the absence and presence of salt stress. Plants from 20 days after sowing (6 days after salt treatment) were used for RNA extraction and hybridization on Affymetrix microarrays. There were two biological replicates for each genotype and salt treatment combination.
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| Contributor(s) |
Chan Z, Grumet R, Loescher W |
| Citation(s) |
21821598 |
| Submission date |
Sep 22, 2009 |
| Last update date |
Jun 12, 2017 |
| Contact name |
Zhulong Chan |
| E-mail(s) |
chanzhul@msu.edu
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| Organization name |
Wuhan Botanic Garden, Chinese Academy of Sciences
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| Street address |
Wuchang District, Moshan
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| City |
Wuhan |
| State/province |
Hubei |
| ZIP/Postal code |
430074 |
| Country |
China |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (8)
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| GSM455422 |
M6PR transgenic_leaf_0mM NaCl_rep1 |
| GSM455423 |
M6PR transgenic_leaf_0mM NaCl_rep2 |
| GSM455424 |
M6PR transgenic_leaf_100mM NaCl_rep1 |
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| Relations |
| BioProject |
PRJNA119589 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE18217_RAW.tar |
17.2 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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