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| Status |
Public on Nov 24, 2021 |
| Title |
Expression data from the Neural Stem Cells (NSCs) of LPS induced depression mice model treated with Luteolin |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Gene expression profiling reveals a potential role of Luteolin in LPS induced depression model
LPS depression induced mice were orally treated with luteolin (10 mg/kg body weight) once per day during 8 consecutive days. Microarray gene expression was conducted for isolated mice Neural Stem Cells (NSCs) . Two mice brain were used for each expermient. The Microarray gene expression was conducted on NSCs and hipocampus from LPS depression induced depression mice (LPS group), LPS depression induced depression mice treated with luteolin(LPS+L group), Normal mice (PBS group) and Normal mice treated with luteolin (PBS+L).
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| Overall design |
RNA was extracted using Isogen (Nippon Gene Co. Ltd., Toyama, Japan). The integrity of RNA was quantified using NanoDrop 2000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA). RNA samples were prepared for gene expression profiling analysis with GeneChip® 3' Expression Arrays using 3' IVT PLUS Reagent Kit (Affymetrix Inc., Santa Clara, CA, USA). One hundred ng of total RNA from each sample was used to generate amplified and biotinylated complementary RNA (cRNA) from poly (A) RNA in a total RNA sample according to the user manual. IVT Incubation time was 16 hour. GeneAtlas® Hybridization, Wash and Stain Kit was used for hybridizing 3' IVT Array Strips according to the user manual (P/N 08-0306). Mouse genome array strips (MG-430) were hybridized for 16 hours in a 45oC incubator, washed and stained and finally imaging was done with the GeneAtlas Fluidics and Imaging Station.
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| Contributor(s) |
ACHOUR M |
| Citation(s) |
34790666 |
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| Submission date |
Aug 05, 2021 |
| Last update date |
Nov 25, 2021 |
| Contact name |
Mariem Achour |
| E-mail(s) |
achourmariem@gmail.com
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| Organization name |
Alliance for Research on the Mediterranean and North Africa
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| Street address |
1-1-1 Tennodai
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| City |
Tsukuba |
| State/province |
Ibaraki |
| ZIP/Postal code |
305-8572 |
| Country |
Japan |
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| Platforms (1) |
| GPL11180 |
[HT_MG-430_PM] Affymetrix HT MG-430 PM Array Plate |
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| Samples (7)
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| Relations |
| BioProject |
PRJNA752354 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE181522_RAW.tar |
9.2 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| GSE181522_additional_processed_data.xls.gz |
16.3 Mb |
(ftp)(http) |
XLS |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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