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| Status |
Public on Nov 01, 2023 |
| Title |
Effect of anti-CD20 treatment on the composition and gene expression of meningeal ectopic lymphoid tissue and CSFin a spontaneous EAE model, using single cell gene expression . |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Objective: To investigate how anti-CD20 B cell-depleting monoclonal antibodies (ɑCD20 mAb) alter the composition and gene expression of immune cells in meningeal ectopic lymphoid tissue (mELT) and the CSF in a murine model of Multiple Sclerosis (MS). Methods: We utilized a spontaneous chronic experimental autoimmune encephalomyelitis (EAE) model of mice with mutant T and B cell receptors specific for myelin oligodendrocyte glycoprotein (MOG), which develop meningeal inflammatory infiltrates resembling those described in Multiple Sclerosis (MS). We studied the changes in cell composition and gene expression after anti-CD20 treatment in mELT and CSF.
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| Overall design |
single cell gene expression and Bcell receptor sequences of meningeal ectopic lymphoid tissue and CSF in 3 anti-CD20 and 3 vehicle treated Thx2D2 mice
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| Contributor(s) |
Lepennetier G, Lehmann-Horn K, Diddens J, Georgieva T, Verena F |
| Citation(s) |
38933267 |
| |
| Submission date |
Aug 05, 2021 |
| Last update date |
Jul 31, 2024 |
| Contact name |
Gildas Lepennetier |
| E-mail(s) |
gildas.lepennetier@tum.de
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| Organization name |
Klinikum Rechts der Isar
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| Department |
Neurology
|
| Street address |
Ismaninger Straße 22
|
| City |
Munich |
| ZIP/Postal code |
81675 |
| Country |
Germany |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA752282 |
| SRA |
SRP331195 |
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