GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE181467 Query DataSets for GSE181467
Status Public on Apr 22, 2022
Title mRNA sequencing of SKMEL28 cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary A RNA-seq analysis was performed to characterize the molecular intermediates responsible for the deleterious effects upon DUSP4 loss in SKMEL28 cells. Methods:SKMEL28 cells were transfected with siRNA against DUSP4 or non-targeting control with or without Trametinib (Tram) at 0.25nM. After 16 hours, cell were collected and RNAseq experiments were performed. Results: the expression of MITF and some of its target genes were significantly downregulated in the absence of DUSP4 and completely rescued by trametinib treatment. Conclusions: In mutant melanoma cells, the expression of MITF and its related target genes is subject to regulation by the DUSP4-ERK axis.
Overall design Examination of mRNA profiles of siNT and siDUSP4 SKMEL28 cells treated with or without 0.25nM Trametinib for 16h
Contributor(s) Prat NG, Karimaddini Z, Wolf L, Tyanova S, Wellinger LC, Huberer HL, Marbach D, Griesser V, Pettazzoni P, Bischoff JR, Rohle D, Palladino C, Vivanco I
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Aug 04, 2021
Last update date Apr 22, 2022
Contact name Daniel Marbach
Phone 0789663168
Organization name F. Hoffmann - La Roche Ltd
Department pRED Pharmaceutical Sciences
Street address Grenzacherstrasse 124
City Basel
ZIP/Postal code 4070
Country Switzerland
Platforms (1)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
Samples (12)
GSM5503004 1_16h_SKMEL28_SCR_1
GSM5503005 2_16h_SKMEL28_SCR_2
GSM5503006 3_16h_SKMEL28_SCR_3
BioProject PRJNA752118
SRA SRP331151

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE181467_raw_gene_counts.gct.gz 640.7 Kb (ftp)(http) GCT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap