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| Status |
Public on Apr 22, 2022 |
| Title |
mRNA sequencing of SKMEL28 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
A RNA-seq analysis was performed to characterize the molecular intermediates responsible for the deleterious effects upon DUSP4 loss in SKMEL28 cells. Methods:SKMEL28 cells were transfected with siRNA against DUSP4 or non-targeting control with or without Trametinib (Tram) at 0.25nM. After 16 hours, cell were collected and RNAseq experiments were performed. Results: the expression of MITF and some of its target genes were significantly downregulated in the absence of DUSP4 and completely rescued by trametinib treatment. Conclusions: In mutant melanoma cells, the expression of MITF and its related target genes is subject to regulation by the DUSP4-ERK axis.
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| Overall design |
Examination of mRNA profiles of siNT and siDUSP4 SKMEL28 cells treated with or without 0.25nM Trametinib for 16h
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| Contributor(s) |
Prat NG, Karimaddini Z, Wolf L, Tyanova S, Wellinger LC, Huberer HL, Marbach D, Griesser V, Pettazzoni P, Bischoff JR, Rohle D, Palladino C, Vivanco I |
| Citation missing |
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| Submission date |
Aug 04, 2021 |
| Last update date |
Apr 22, 2022 |
| Contact name |
Daniel Marbach |
| E-mail(s) |
daniel.marbach@gmail.com
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| Phone |
0789663168
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| Organization name |
F. Hoffmann - La Roche Ltd
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| Department |
pRED Pharmaceutical Sciences
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| Street address |
Grenzacherstrasse 124
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| City |
Basel |
| ZIP/Postal code |
4070 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA752118 |
| SRA |
SRP331151 |
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