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| Status |
Public on Nov 18, 2021 |
| Title |
Chronic IL-1 Exposed AR+ PCa Cell Lines Show Conserved Loss of IL-1 Sensitivity and Evolve Both Conserved and Unique Differential Gene Expression Profiles |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
AR+ PCa cell lines show conserved activation of canonical IL-1 intracellular signaling cascades, including repression of androgen receptor mRNA levels, in response to acute IL-1 treatment and show conserved loss of sensitivity to prolonged (chronic) IL-1 intracelluar signaling, including the restoration of the androgen receptor. Thus, chronic IL-1 exposure selects for cells that have a growth advantage against cytotoxic/static inflammation, including restoration of constitutive AR expression.
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| Overall design |
MDA-PCa-2b cells were chronically cultured in 0.5 ng/ml IL-1 alpha or IL-1 beta (in HPC1 + 20% FBS) for 4 months. IL-1 is cytotoxic/static, so the viable, proliferating colonies that emerged after 4 months in IL-1 alpha or IL-1 beta were expanded to generate the IL-1a subline (MDA-αs1) and the IL-1b subline (MDA-βs1). MDA-PCa-2b parental cells were cultured in vehicle (0.1% BSA in 1xPBS) in HPC1 + 20% FBS. For basal expression and acute IL-1 treatment expression studies, MDA-PCa-2b parental, MDA-αs1 and MDA-βs1 cells were cultured in vehicle (0.1% BSA in 1xPBS) in HPC1 + 20% FBS for 3 days and the MDA-PCa-2b parental cells were treated with 25 ng/ml IL-1 alpha or IL-1 beta in HPC1 + 20% FBS for 3 days. Total RNA was isolated from 3 biological replicates for RNA sequencing.
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| Contributor(s) |
Thomas-Jardin S, Kanchwala M, Dahl H, Liu V, Ahuja R, Soundharrajan R, Roos N, Xing C, Delk NA |
| Citation(s) |
34988553 |
| |
| Submission date |
Jul 30, 2021 |
| Last update date |
Jan 11, 2022 |
| Contact name |
Nikki Delk |
| E-mail(s) |
nad140230@utdallas.edu
|
| Phone |
9728832581
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| Organization name |
University of Texas at Dallas
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| Department |
Biological Sciences
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| Street address |
800 West Campbell Road
|
| City |
Richardson |
| ZIP/Postal code |
75080 |
| Country |
USA |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA751088 |
| SRA |
SRP330625 |
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