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| Status |
Public on Aug 25, 2021 |
| Title |
Fate mapping of single NK cells identifies a type 1 innate lymphoid-like lineage that bridges innate and adaptive recognition of viral infection [bulk_RNAseq_steady_state] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Upon viral infection, NK cells expressing certain germline-encoded receptors are selected, expanded and maintained in an adaptive-like manner. Currently, these are thought to differentiate along a common pathway. However, by fate mapping of single NK cells upon murine cytomegalovirus (MCMV) infection, we identified two distinct NK cell lineages that contributed to adaptive-like responses. One was equivalent to conventional NK (cNK) cells while the other was transcriptionally similar to type 1 innate lymphoid cells (ILC1s). ILC1-like NK cells showed splenic-residency and strong cytokine production but also recognized and killed MCMV-infected cells, guided by activating receptor Ly49H. Moreover, they induced clustering of conventional type 1 dendritic cells and facilitated antigen-specific T cell priming early during MCMV infection, which depended on Ly49H and the NK cell-intrinsic expression of transcription factor Batf3. Thereby, ILC1-like NK cells bridge innate and adaptive viral recognition and unite critical features of cNK cells and ILC1s.
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| Overall design |
4 distinct Ly49H+ NK cell subsets defined by differential expression of CD27 and CD62L as well as ILC1s were sorted from spleen of uninfected C57BL/6 mice. 2x 500 cells were sorted from each population and bulk RNA-Seq was performed of all samples.
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| Contributor(s) |
Flommersfeld S, Böttcher JP, Ersching J, Flossdorf M, Meiser P, Pachmayr LO, Leube J, Hensel I, Jarosch S, Zhang Q, Chaudhry MZ, Andrä I, Schiemann M, Busch DH, Cicin-Sain L, Sun JC, Gasteiger G, Victora GD, Höfer T, Buchholz VR, Grassmann S |
| Citation(s) |
34437840 |
| |
| Submission date |
Jul 27, 2021 |
| Last update date |
Sep 02, 2021 |
| Contact name |
Sophie Flommersfeld |
| E-mail(s) |
sophie.flommersfeld@tum.de
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| Organization name |
Technical University of Munich
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| Department |
Institute for Medical Microbiology, Immunology and Hygiene
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| Lab |
Veit Buchholz
|
| Street address |
Trogerstr. 30
|
| City |
München |
| ZIP/Postal code |
81675 |
| Country |
Germany |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (8)
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| GSM5481644 |
CD27- CD62L+ Ly49H+ NK_spleen_steady state_1 |
| GSM5481645 |
CD27- CD62L+ Ly49H+ NK_spleen_steady state_2 |
| GSM5481646 |
CD27+ CD62L+ Ly49H+ NK_spleen_steady state_1 |
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| This SubSeries is part of SuperSeries: |
| GSE180978 |
Fate mapping of single NK cells identifies a type 1 innate lymphoid-like lineage that bridges innate and adaptive recognition of viral infection |
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| Relations |
| BioProject |
PRJNA750152 |
| SRA |
SRP330107 |
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