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| Status |
Public on Apr 06, 2023 |
| Title |
Harnessing matrix stiffness to engineer a bone marrow niche for hematopoietic stem cell rejuvenation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
RNA and ATAC sequencing data of primary sorting CD45-Ter119-CD31-Scf; GFP+Cxcl12; DsRed+ bone marrow stromal cells ,2D cultured bone marrow stromal cells and 3D cultured bone marrow stromal cells. RNA sequencing data of sorted primary and 3D cocultured Lin-Sca1+C-kit+CD150+CD48+ hematopoietic stem cells from 8-12 weeks and 12-13 months old mice.
RNA and ATAC sequencing data of primary sorting CD45-Ter119-CD31-Pdgfra+td-Tomato+ bone marrow stromal cells from young (8 wks), middle aged (12 months) and aged (22-24 months) Lepr-Cre;td-Tomato mice.
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| Overall design |
Total RNA from 3 biological replicates of CD45-Ter119-CD31-Scf; GFP+Cxcl12; DsRed+ bone marrow stromal cells,2D cultured bone marrow stromal cells and 3D cultured bone marrow stromal cells. Paired-end sequencing 150bp reads Novaseq (Illumina). ATAC-seq of 3 biological replicates of CD45-Ter119-CD31-Scf; GFP+Cxcl12; DsRed+ bone marrow stromal cells,2D cultured bone marrow stromal cells and 3D cultured bone marrow stromal cells.Paired-end sequencing 150bp reads HiSeq X Ten system (Illumina). RNA-seq of 3 biological replicates of sorted primary and 3D cocultured Lin-Sca1+C-kit+CD150+CD48+ hematopoietic stem cells from 8-12 weeks and 12-13 months old mice.Paired-end sequencing 150bp reads HiSeq X Ten system (Illumina).
Total RNA from 3 biological replicates of CD45-Ter119-CD31-Pdgfra+td-Tomato+ bone marrow stromal cells from young (8 wks), middle aged (12 months) and aged (22-24 months) Lepr-Cre;td-Tomato mice. Paired-end sequencing 150bp reads Novaseq (Illumina). ATAC-seq of 3 biological replicates of CD45-Ter119-CD31-Pdgfra+td-Tomato+ bone marrow stromal cells from young (8 wks), middle aged (12 months) and aged (22-24 months) Lepr-Cre;td-Tomato mice. Paired-end sequencing 150bp reads (Illumina).
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| Web link |
https://doi.org/10.1016/j.stem.2023.03.005
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| Contributor(s) |
Yue R, Zhang X, Cao D, Xu L |
| Citation(s) |
37028404 |
| |
| Submission date |
Jul 26, 2021 |
| Last update date |
Apr 08, 2023 |
| Contact name |
Dandan Cao |
| E-mail(s) |
1931465@tongji.edu.cn
|
| Phone |
17853136996
|
| Organization name |
Tongji University
|
| Street address |
1239 Siping Road, Yangpu District
|
| City |
Shanghai |
| ZIP/Postal code |
200092 |
| Country |
China |
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| Platforms (2) |
| GPL21273 |
HiSeq X Ten (Mus musculus) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (41)
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| Relations |
| BioProject |
PRJNA749784 |
| SRA |
SRP329936 |
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