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Series GSE180647 Query DataSets for GSE180647
Status Public on Jul 29, 2021
Title Dendritic cells maintain anti-tumor immunity by positioning CD8 resident memory T cells in the skin through the CXCL16-CXCR6 axis
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Tissue-resident memory (TRM) T cells are emerging as critical components of the immune response to cancer; yet, requirements for their ongoing function and maintenance remain unclear. Antigen presenting cells (APC) promote TRM cell differentiation and re-activation but have not been implicated in sustaining TRM cell responses. Here, we identified a novel role for dendritic cells in supporting tissue resident memory to melanoma. We showed that CD8 TRM cells remain in close proximity to dendritic cells in the skin. Depletion of CD11c+ cells results in rapid disaggregation and eventual loss of melanoma-specific TRM cells. Additionally, we determined that TRM migration and/or persistence requires chemotaxis and adhesion mediated by the CXCR6/CXCL16 axis. The interaction between CXCR6-expressing TRM cells and CXCL16-expressing APCs was found to be critical for sustaining TRM cell-mediated tumor protection. These findings substantially expand our knowledge of APC functions in tissue resident memory T cell homeostasis and longevity.
Overall design comparing CD8 T cells, CD11c+CD11bneg and CD11c+CD11b+ monocytes from melanoma associated vitiligo skin of mice
Contributor(s) Vella JL, Molodtsov A, Angeles CV, Angeles CV, Turk MJ, Huang YH
Citation(s) 34362825
Submission date Jul 22, 2021
Last update date Aug 31, 2021
Contact name Jennifer Vella
Organization name Dartmouth College
Street address 1 medical center drive
City lebanon
ZIP/Postal code 03755
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (9)
GSM5466867 CD11c+CD11bneg (rep 1)
GSM5466868 CD11c+CD11b+ (rep 1)
GSM5466869 CD8 T cells (rep 1)
BioProject PRJNA749023
SRA SRP329465

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Supplementary file Size Download File type/resource
GSE180647_featurecounts.readcounts_rpkm.ann.tsv.gz 5.4 Mb (ftp)(http) TSV
GSE180647_featurecounts.readcounts_tpm.ann.tsv.gz 5.4 Mb (ftp)(http) TSV
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Raw data are available in SRA
Processed data are available on Series record

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