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| Status |
Public on Jul 12, 2022 |
| Title |
seRNA PAM-1 interacts with Ddx5 to regulate skeletal muscle satellite cell activation and aging through trans regulation of Timp2 expression [PAM-1 ChIRP-seq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
There is an absolute requirement of Pax7 for the normal function of MuSCs during regenerative myogenesis in skeletal muscle at any stage of life. Here using RNA-seq, H3K27ac and Pax7 ChIP-seq, we discover PAM-1 (Pax7 Associated Muscle lncRNA) that is enriched in activated skeletal muscle satellite cells (ASCs) 24 and 48 hours after activation. Knockdown of PAM-1 reduces proliferating Pax7+Myod+ ASCs number, while overexpression of PAM-1 increases ASCs number. Mechanistically, PAM-1 is located on ASCs and myoblast specific super-enhancer (SE), and we categorize it as seRNA. Through a series of multiomics analysis of PAM-1 interactome in myoblast including PAM-1-DNA interaction by ChIRP-seq, PAM-1 SE-DNA interaction by 4C-seq, PAM-1-protein interaction by mass spectrometry and ChIP-seq, we identify a novel class of transcriptional regulation that seRNA PAM-1 interacts with RNA binding protein Ddx5 and tethers PAM-1 SE to regulate inter-chromosomal targets Timp2. Altogether, our findings identify PAM-1 is driven by Pax7 in ASC and myoblast to regulate myogenic activation through binding with Ddx5 and targeting Timp2.
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| Overall design |
ChIRP-seq on PAM-1 seRNA
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| |
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| Contributor(s) |
So KK, Huang Y, Sun H, Wang H |
| Citation(s) |
35851988 |
| |
| Submission date |
Jul 14, 2021 |
| Last update date |
Aug 24, 2022 |
| Contact name |
Yile Huang |
| E-mail(s) |
huangyilekuaile@gmail.com
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| Phone |
56289467
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| Organization name |
The Chinese University of HongKong
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| Department |
Chemical Pathology
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| Street address |
Room 503, Li Ka Shing Health and Science Buiding, Prince of Wales Hospital, Sha Tin
|
| City |
Sha Tin |
| State/province |
Hong Kong |
| ZIP/Postal code |
999077 |
| Country |
China |
| |
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| Platforms (1) |
| GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
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| Samples (3) |
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| This SubSeries is part of SuperSeries: |
| GSE180073 |
seRNA PAM-1 interacts with Ddx5 to regulate skeletal muscle satellite cell activation and aging through trans regulation of Timp2 expression |
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| Relations |
| BioProject |
PRJNA746467 |
| SRA |
SRP328286 |
