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| Status |
Public on Jul 13, 2024 |
| Title |
Redox-associated miRNAs regulated by anti-TNBC compounds DET and DETD-35 in MDA-MB-231 cells |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
Triple-negative breast cancer is a difficult breast cancer subtype to treat that lacks targeted therapies. Specific miRNAs have been reported to be involved in cancer progression and their significance in cancer diagnosis and anti-cancer drug development has been highlighted; however, whether redox-associated miRNAs are potential targets of anti-cancer drugs or bioactive phytocompounds, which deregulate cancer cell activity through modulating ROS dynamics is unclear. This study addressed the anti-TNBC cell effect of phytosesquiterpene lactones deoxyelephantopin (DET) and its derivative DETD-35 by which redox-associated miRNAs were responsive and regulated as demonstrated by comparative miRNA microarray analysis and ROS scavenging approaches.
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| Overall design |
MDA-MB-231 cells were incubated with vehicle (0.5% DMSO), DET (4 µM) or DETD-35 (4 µM) for 1 h, with or without pretreatment of antioxidant N-acetylcysteine (NAC, 5 mM, 1 h) prior to RNA extraction and analysis. Two technical repeats by using two independent arrays were carried out for each sample. miRNA expression levels in vehicle treatment group was used to normalize the data in DET and DETD-35 treatment groups. Similarly, miRNA expressions in NAC-pretreated vehicle group was used to normalize the data in NAC-pretreated DET and DETD-35 groups. A selection criterion of P < 0.05 and log2(ratio over vehicle) < -0.8 or log2(ratio over vehicle) > 0.8 was used to identify specific miRNAs upregulated or downregulated by DET or DETD-35 treatment compared to vehicle group, and to evaluate whether regulation of specific miRNAs by DET or DETD-35 can be affected by NAC pretreatment.
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| Contributor(s) |
Shyur L, Cheng Y |
| Citation missing |
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| Submission date |
Jul 13, 2021 |
| Last update date |
Jul 13, 2024 |
| Contact name |
Lie-Fen Shyur |
| E-mail(s) |
lfshyur@ccvax.sinica.edu.tw
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| Organization name |
Academia Sinica
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| Street address |
No. 128, Sec. 2, Academia Road
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| City |
Taipei |
| ZIP/Postal code |
115 |
| Country |
Taiwan |
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| Platforms (1) |
| GPL30415 |
Phalanx Human miRNA OneArray v7.2 |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA746246 |
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