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Series GSE180000 Query DataSets for GSE180000
Status Public on Jul 13, 2024
Title Redox-associated miRNAs regulated by anti-TNBC compounds DET and DETD-35 in MDA-MB-231 cells
Organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary Triple-negative breast cancer is a difficult breast cancer subtype to treat that lacks targeted therapies. Specific miRNAs have been reported to be involved in cancer progression and their significance in cancer diagnosis and anti-cancer drug development has been highlighted; however, whether redox-associated miRNAs are potential targets of anti-cancer drugs or bioactive phytocompounds, which deregulate cancer cell activity through modulating ROS dynamics is unclear. This study addressed the anti-TNBC cell effect of phytosesquiterpene lactones deoxyelephantopin (DET) and its derivative DETD-35 by which redox-associated miRNAs were responsive and regulated as demonstrated by comparative miRNA microarray analysis and ROS scavenging approaches.
 
Overall design MDA-MB-231 cells were incubated with vehicle (0.5% DMSO), DET (4 µM) or DETD-35 (4 µM) for 1 h, with or without pretreatment of antioxidant N-acetylcysteine (NAC, 5 mM, 1 h) prior to RNA extraction and analysis. Two technical repeats by using two independent arrays were carried out for each sample. miRNA expression levels in vehicle treatment group was used to normalize the data in DET and DETD-35 treatment groups. Similarly, miRNA expressions in NAC-pretreated vehicle group was used to normalize the data in NAC-pretreated DET and DETD-35 groups. A selection criterion of P < 0.05 and log2(ratio over vehicle) < -0.8 or log2(ratio over vehicle) > 0.8 was used to identify specific miRNAs upregulated or downregulated by DET or DETD-35 treatment compared to vehicle group, and to evaluate whether regulation of specific miRNAs by DET or DETD-35 can be affected by NAC pretreatment.
 
Contributor(s) Shyur L, Cheng Y
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Submission date Jul 13, 2021
Last update date Jul 13, 2024
Contact name Lie-Fen Shyur
E-mail(s) lfshyur@ccvax.sinica.edu.tw
Organization name Academia Sinica
Street address No. 128, Sec. 2, Academia Road
City Taipei
ZIP/Postal code 115
Country Taiwan
 
Platforms (1)
GPL30415 Phalanx Human miRNA OneArray v7.2
Samples (12)
GSM5444694 vehicle_technical repeat 1
GSM5444695 vehicle_technical repeat 2
GSM5444696 NAC+vehicle_technical repeat 1
Relations
BioProject PRJNA746246

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE180000_RAW.tar 5.4 Mb (http)(custom) TAR (of GPR)
Processed data included within Sample table

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