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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jul 08, 2021 |
| Title |
Direct reprogramming of fibroblasts into a cardiovascular tissue |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: To explore detailed transcriptome changes during the transition from fibroblasts toward cardiac cells
Methods: We performed single-cell RNA-seq on MTTFs (11,580 cells) and MTTFs treated with MAB for 6 days (12,261 cells). In MTTFs, the mean reads and mediam genes per cell were 53,107 and 5,096, respectively; In reprogrammed cells, the mean reads and medium genes per cell were 100,515 and 6,164, respectively.
Result & Conclusion: Single-cell RNA-seq studies demonstrate a simultaneous reprogramming of mouse tail-tip fibroblasts (MTTFs) by MAB into CMs, Ecs and SMCs.
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| Overall design |
single-cell RNA-seq on MTTFs (11,580 cells) and MTTFs treated with MAB for 6 days
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| Contributor(s) |
Cho J, Yoon Y |
| Citation(s) |
34426676 |
| Submission date |
Jul 06, 2021 |
| Last update date |
Feb 08, 2022 |
| Contact name |
Jaeyeaon Cho |
| E-mail(s) |
jaeyeaon@gmail.com
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| Organization name |
Yonsei University College of Medicine
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| Street address |
Yonsei-ro 50
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| City |
seoul |
| ZIP/Postal code |
03722 |
| Country |
South Korea |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA744237 |
| SRA |
SRP327197 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE179589_RAW.tar |
456.0 Mb |
(http)(custom) |
TAR (of CLOUPE) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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