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Series GSE179023 Query DataSets for GSE179023
Status Public on Aug 10, 2021
Title RNA-sequencing reveals niche gene expression effects of beta-hydroxybutyrate in primary myotubes
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Various forms of fasting, including time-restricted feeding, alternate day fasting, and periodic fasting have shown promise in clinical and pre-clinical studies to normalize body weight, improve metabolic health, and protect against disease. Recent studies suggest that β-hydroxybutyrate (βOHB), a characteristic ketone body of the fasted metabolic state, acts as a potential signaling molecule mediating the beneficial effects of the various forms of fasting, potentially by acting as a histone deacetylase inhibitor. In the first part we investigated whether βOHB, in comparison to the well-established histone deacetylase inhibitor butyrate, influences cellular differentiation in vitro. In C2C12 myotubes, 3T3-L1 adipocytes, and THP-1 monocytes, millimolar concentrations of βOHB did not alter differentiation, as determined by gene expression and histological assessment, whereas equimolar concentrations of butyrate potently impaired differentiation in all cell types. RNA-sequencing revealed that unlike butyrate, βOHB minimally impacted gene expression in adipocytes, macrophages, and hepatocytes. However, in myocytes, βOHB upregulated genes involved in the TCA cycle and oxidative phosphorylation, while downregulating genes belonging to cytokine and chemokine signal transduction. Overall, our data do not support the notion that βOHB serves as a powerful signaling molecule regulating gene expression in adipocytes, macrophages and hepatocytes, but suggest that βOHB may act as a niche signaling molecule in muscle.
Overall design Mouse primary cells (adipocytes, macrophages, skeletal myotubes, hepatocytes) were exposed to β-hydroxybutyric acid (bOHB; 5mM), butyric acid (5mM), or control treatment, and subjected to gene expression profiling by RNA-sequencing.
Contributor(s) Ruppert PM, Deng L, Hooiveld GJ, Hangelbroek RW, Zeigerer A, Kersten S
Citation(s) 34407998
Submission date Jun 28, 2021
Last update date Aug 24, 2021
Contact name Guido Hooiveld
Organization name Wageningen University
Department Div. Human Nutrition & Health
Lab Nutrition, Metabolism & Genomics Group
Street address HELIX, Stippeneng 4
City Wageningen
ZIP/Postal code NL-6708WE
Country Netherlands
Platforms (1)
GPL23479 BGISEQ-500 (Mus musculus)
Samples (36)
GSM5403816 primary adipocytes, control treatment, replicate 1
GSM5403817 primary adipocytes, control treatment, replicate 2
GSM5403818 primary adipocytes, control treatment, replicate 3
BioProject PRJNA741937
SRA SRP325858

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Supplementary file Size Download File type/resource
GSE179023_TxImport.GeneLevel.counts.GEO.txt.gz 1.8 Mb (ftp)(http) TXT
GSE179023_txi.counts.annotated.GEO.Rdata.gz 19.2 Mb (ftp)(http) RDATA
GSE179023_txi.lengthScaledTPM.annotated.GEO.Rdata.gz 22.3 Mb (ftp)(http) RDATA
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Raw data are available in SRA
Processed data are available on Series record

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